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First published online 7 March 2006
doi: 10.1242/jcs.02847

Journal of Cell Science 119, 1255-1265 (2006)
Published by The Company of Biologists 2006
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Research Article

The plasma membrane recycling pathway and cell polarity in plants: studies on PIN proteins

Yohann Boutté1, Marie-Thérèse Crosnier1, Nicola Carraro2, Jan Traas2 and Béatrice Satiat-Jeunemaitre1,*

1 Laboratoire de Dynamique de la Compartimentation Cellulaire, Institut des Sciences du Végétal, CNRS UPR2355, 9 Gif-sur-Yvette CEDEX, France
2 Laboratoire de Biologie Cellulaire, INRA, Route de Saint Cyr, 78026 Versailles CEDEX, France

* Author for correspondence (e-mail: bsj{at}isv.cnrs-gif.fr)

Accepted 14 December 2005

The PIN-FORMED (PIN) proteins are plasma-membrane-associated facilitators of auxin transport. They are often targeted to one side of the cell only through subcellular mechanisms that remain largely unknown. Here, we have studied the potential roles of the cytoskeleton and endomembrane system in the localisation of PIN proteins. Immunocytochemistry and image analysis on root cells from Arabidopsis thaliana and maize showed that 10-30% of the intracellular PIN proteins mapped to the Golgi network, but never to prevacuolar compartments. The remaining 70-90% were associated with yet to be identified structures. The maintenance of PIN proteins at the plasma membrane depends on a BFA-sensitive machinery, but not on microtubules and actin filaments.

The polar localisation of PIN proteins at the plasmamembrane was not reflected by any asymmetric distribution of cytoplasmic organelles. In addition, PIN proteins were inserted in a symmetrical manner at both sides of the cell plate during cytokinesis. Together, the data indicate that the localisation of PIN proteins is a postmitotic event, which depends on local characteristics of the plasma membrane and its direct environment. In this context, we present evidence that microtubule arrays might define essential positional information for PIN localisation. This information seems to require the presence of an intact cell wall.

Key words: Plant cell polarity, PIN proteins, Endomembrane, Cytoskeleton, Immunocytochemistry, Confocal microscopy






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