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First published online 14 March 2006
doi: 10.1242/jcs.02862

Journal of Cell Science 119, 1341-1349 (2006)
Published by The Company of Biologists 2006
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Research Article

Role of neuropsin in formation and maturation of Schaffer-collateral L1cam-immunoreactive synaptic boutons

Yukiko Nakamura, Hideki Tamura, Kazuhiro Horinouchi and Sadao Shiosaka*

Division of Structural Cell Biology, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0192, Japan

* Author for correspondence (e-mail: sshiosak{at}bs.naist.jp)

Accepted 19 December 2005

We report that neuropsin is involved in the synaptogenesis/maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway. Most non-synaptic orphan boutons and a number of immature small synaptic boutons expressed the cell adhesion molecule L1 in presynaptic Schaffer-collateral terminals, whereas mature large boutons on mushroom spines were devoid of L1. The number of L1-immunoreactive boutons was markedly higher in neuropsin-deficient mice than in wild-type mice, whereas there were far fewer mature large boutons. L1-immunoreactive boutons were hypertrophied in the mutant mice. When a recombinant active neuropsin was microinjected into the mutant hippocampus, the number of immunoreactive synaptic boutons reverted to wild-type levels after one day. These results strongly suggest that enzymatically active neuropsin allows a maturational change of L1-immunoreactive small boutons, both orphan and synaptic, and this step may be important in synaptic plasticity based on activity-dependent structural change.

Key words: KLK8, Serine protease, Cell adhesion molecule, Synapse, Orphan bouton, Synaptogenesis


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 J. Neurosci.Home page
Y. Ishikawa, Y. Horii, H. Tamura, and S. Shiosaka
Neuropsin (KLK8)-Dependent and -Independent Synaptic Tagging in the Schaffer-Collateral Pathway of Mouse Hippocampus
J. Neurosci., January 23, 2008; 28(4): 843 - 849.
[Abstract] [Full Text] [PDF]


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