spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 14 March 2006
doi: 10.1242/jcs.02839

Journal of Cell Science 119, 1361-1370 (2006)
Published by The Company of Biologists 2006
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow All Versions of this Article:
jcs.02839v1
119/7/1361    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Phillips, M. D.
Right arrow Articles by Thomas, G. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Phillips, M. D.
Right arrow Articles by Thomas, G. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Brush border spectrin is required for early endosome recycling in Drosophila

Matthew D. Phillips* and Graham H. Thomas{ddagger}

Departments of Biology, and of Biochemistry and Molecular Biology, The Pennsylvania State University, 208 Erwin W. Mueller Laboratory, University Park, PA 16802, USA

{ddagger} Author for correspondence (e-mail: gxt5{at}psu.edu)

Accepted 15 December 2005

An apical brush border is a characteristic of many mature epithelia. This dynamic structure consists of dense microvilli supported by F-actin bundles that protrude into the apical cytoplasm, where they are crosslinked by spectrin and myosin II to form the terminal web. Little is known about the terminal web, through which vesicles transit to and from the apical membrane. Analysis of mutations in ßHeavy-spectrin, the Drosophila brush border spectrin, reveals that this protein is necessary for the maintenance of Rab5 endosomes in the midgut. As a consequence, an apical H+ V-ATPase that is probably responsible for lumenal acidification is lost both from the brush border and Rab5 endosomes. Epistasis tests indicate that ßHeavy-spectrin is required during endocytosis after Dynamin and before Rab5-mediated endosome activities. These data are consistent with the location of spectrin in the terminal web, and suggest that this molecule is required for correct sorting decisions at the early endosome.

Key words: Epithelium, Brush border, Spectrin, Rab GTPase, Endosome, Drosophila


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in JCS:

The spectrin haunting endocytosis

JCS 2006 119: 702. [Full Text]  



This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. Das, C. Base, D. Manna, W. Cho, and R. R. Dubreuil
Unexpected Complexity in the Mechanisms That Target Assembly of the Spectrin Cytoskeleton
J. Biol. Chem., May 2, 2008; 283(18): 12643 - 12653.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006