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First published online 14 March 2006
doi: 10.1242/jcs.02839

Journal of Cell Science 119, 1361-1370 (2006)
Published by The Company of Biologists 2006
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Research Article

Brush border spectrin is required for early endosome recycling in Drosophila

Matthew D. Phillips* and Graham H. Thomas{ddagger}

Departments of Biology, and of Biochemistry and Molecular Biology, The Pennsylvania State University, 208 Erwin W. Mueller Laboratory, University Park, PA 16802, USA

{ddagger} Author for correspondence (e-mail: gxt5{at}psu.edu)

Accepted 15 December 2005

An apical brush border is a characteristic of many mature epithelia. This dynamic structure consists of dense microvilli supported by F-actin bundles that protrude into the apical cytoplasm, where they are crosslinked by spectrin and myosin II to form the terminal web. Little is known about the terminal web, through which vesicles transit to and from the apical membrane. Analysis of mutations in ßHeavy-spectrin, the Drosophila brush border spectrin, reveals that this protein is necessary for the maintenance of Rab5 endosomes in the midgut. As a consequence, an apical H+ V-ATPase that is probably responsible for lumenal acidification is lost both from the brush border and Rab5 endosomes. Epistasis tests indicate that ßHeavy-spectrin is required during endocytosis after Dynamin and before Rab5-mediated endosome activities. These data are consistent with the location of spectrin in the terminal web, and suggest that this molecule is required for correct sorting decisions at the early endosome.

Key words: Epithelium, Brush border, Spectrin, Rab GTPase, Endosome, Drosophila


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JCS 2006 119: 702. [Full Text]  



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