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Journal of Cell Science, Vol 12, 421-443, Copyright © 1973 by Company of Biologists

Submitted on June 13, 1972

Pollen Wall Proteins: Pollen-Stigma Interactions in Ragweed and Cosmos (Compositae)

R. B. KNOX 1

1 Botany Department, Australian National University, P.O. Box 4, Canberra A.C.T., Australia

The release of wall-held materials from the pollen of ragweed (Ambrosia tenuifolia) and Cosmos bipinnatus on to the stigma surface has been followed. When fresh stigmas were viewed by scanning electron microscopy, a fluid material was observed coating pollen grains, pollen tubes and adjacent stigmatic papillae. This fluid contained pollen-wall antigens, including the allergen Antigen E, detected by immunofluorescence, and proteins, lipids and carbohydrates detected by cytochemical methods.

In Cosmos, the fate of the antigens was much the same after both compatible and incompatible matings. In incompatible matings, pollen tubes were blocked with a polysaccharide considered to be callose. Callose particles appeared on neighbouring stigmatic papillae, and adjacent stigmatic hairs accumulated callose internally. This reaction may be an important one for the incompatibility response.

Submitted on June 13, 1972




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© The Company of Biologists Ltd 1973