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Journal of Cell Science, Vol 12, 603-615, Copyright © 1973 by Company of Biologists

Submitted on June 28, 1972

Association of the Two Glycosyl Transferase Activities of Glycoprotein Synthesis with Low Equilibrium Density Smooth Microsomes

R. R. WAGNER 1, ELISABETH PETTERSSON 1, and G. DALLNER 1

1 Department of Biochemistry, University of Stockholm, and Department of Pathology at Sabbatsberg Hospital, Karolinska Institutet, Stockholm, Sweden

Smooth microsomes of rat liver were subfractionated on a continuous sucrose gradient to isopycnic equilibrium density. All the microsomal electron-transport enzymes and phosphatases exhibit a broad distribution in the various microsomal subfractions. On the other hand, the N-acetylglucosaminyl and galactosyl transferase activities, which are known to participate in the synthesis of the oligosaccharide chain of the plasma glycoproteins, exhibit a markedly different distribution pattern. They are concentrated to a small fraction having an isopycnic equilibrium density of 1.10. These membranes represent a separate entity of the smooth microsomal fraction, the exact cellular origin of which remains to be clarified.

Submitted on June 28, 1972






© The Company of Biologists Ltd 1973