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Journal of Cell Science, Vol 12, 631-639, Copyright © 1973 by Company of Biologists

Submitted on June 23, 1972

Abolition by Myosin and Heavy Meromyosin of the Inhibitory Effect of Smooth-Muscle Actomyosin Antibodies on Cell Aggregation In Vitro

R. B. KEMP 1, B. M. JONES 1, and U. GRÖSCHEL-STEWART 2

1 Department of Zoology, University College of Wales, Aberystwyth, Wales, U.K.
2 Universitäts-Frauenklinik, Würzburg, West Germany

The ability of anti-chicken smooth-muscle actomyosin {gamma}-globulins (anti-GAM) to inhibit the aggregation of dissociated cells from the skeletal muscle and liver of chick embryos was abolished by pretreatment of the anti-GAM with either myosin or heavy meromyosin (HMM). When the same cells were treated with HMM at a concentration of 1 mg per 2 x 106 cells/ml Eagle's MEM they aggregated as readily as untreated cells.

The negative electrophoretic mobility of the embryonic chick fibroblastic cells was significantly reduced by the globulin fraction of anti-GAM but not of HMM-treated anti-GAM or non-immunized rabbit serum. Anti-chicken striated muscle actomyosin {gamma}-globulins slightly reduced negative mobility but HMM had no effect.

The experiments show that the inhibitory effect on cell aggregation of anti-GAM preparations is produced by the anti-myosin antibodies. They also provide support for the theory that a surface-localized myosin-like protein has a regulatory function in cell adhesion.

Submitted on June 23, 1972






© The Company of Biologists Ltd 1973