spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 5 December 2006
doi: 10.1242/jcs.03312

Journal of Cell Science 120, 23-32 (2007)
Published by The Company of Biologists 2007
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.03312v1
120/1/23    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Campalans, A.
Right arrow Articles by Radicella, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Campalans, A.
Right arrow Articles by Radicella, J. P.

Research Article

UVA irradiation induces relocalisation of the DNA repair protein hOGG1 to nuclear speckles

Anna Campalans1,*, Rachel Amouroux1,*, Anne Bravard1, Bernd Epe2 and J. Pablo Radicella1,{ddagger}

1 Département de Radiobiologie et Radiopathologie, Commissariat à l'Energie Atomique, UMR 217 CNRS/CEA, 18 route du Panorama, 92265 Fontenay aux Roses, France
2 Institute of Pharmacy, University of Mainz, 55099 Mainz, Germany

{ddagger} Author for correspondence (e-mail: jpradicella{at}cea.fr)

Accepted 23 October 2006

The DNA glycosylase hOGG1 initiates base excision repair (BER) of oxidised purines in cellular DNA. Using confocal microscopy and biochemical cell fractionation experiments we show that, upon UVA irradiation of human cells, hOGG1 is recruited from a soluble nucleoplasmic localisation to the nuclear matrix. More specifically, after irradiation, hOGG1 forms foci colocalising with the nuclear speckles, organelles that are interspersed between chromatin domains and that have been associated with transcription and RNA-splicing processes. The use of mutant forms of hOGG1 unable to bind the substrate showed that relocalisation of hOGG1 does not depend on the recognition of the DNA lesion by the enzyme. The recruitment of hOGG1 to the nuclear speckles is prevented by the presence of antioxidant compounds during UVA irradiation, implicating reactive oxygen species as signals for the relocalisation of hOGG1. Furthermore, APE1, the second enzyme in the BER pathway, is also present in nuclear speckles in UVA-irradiated cells. The recruitment of DNA repair proteins to nuclear speckles after oxidative stress implicates these organelles in the cellular stress response.

Key words: Base excision repair, hOGG1, Nuclear speckles, Reactive oxygen species, UVA


Related articles in JCS:

Speckles mark the spot for DNA repair

JCS 2007 120: 102. [Full Text]  



This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. Das, R. Chattopadhyay, K. K. Bhakat, I. Boldogh, K. Kohno, R. Prasad, S. H. Wilson, and T. K. Hazra
Stimulation of NEIL2-mediated Oxidized Base Excision Repair via YB-1 Interaction during Oxidative Stress
J. Biol. Chem., September 28, 2007; 282(39): 28474 - 28484.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2007