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First published online 24 April 2007
doi: 10.1242/jcs.03447

Journal of Cell Science 120, 1772-1778 (2007)
Published by The Company of Biologists 2007
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Research Article

Cortical centralspindlin and G{alpha} have parallel roles in furrow initiation in early C. elegans embryos

Koen J. C. Verbrugghe1,2 and John G. White2,3,*

1 Laboratory of Genetics, University of Wisconsin – Madison, Madison, WI 53706, USA
2 Laboratory of Molecular Biology, University of Wisconsin – Madison, Madison, WI 53706, USA
3 Department of Anatomy, University of Wisconsin – Madison, Madison, WI 53706, USA

* Author for correspondence (e-mail: jwhite1{at}wisc.edu)

Accepted 15 March 2007

Evidence from various systems suggests that either asters or the midzone of the mitotic spindle are the predominant determinants of cleavage plane position. Disrupting spindle midzone formation in the one-cell Caenorhabditis elegans embryo, such as by using mutants of the centralspindlin component ZEN-4, prevents completion of cytokinesis but does not inhibit furrowing. However, furrowing is inhibited by the simultaneous depletion of ZEN-4 with either PAR-2 or G{alpha}, which are required for asymmetric divisions. Through studies of other genes required for the presence of an intact spindle midzone containing microtubule bundles, we found that furrowing failed in the absence of PAR-2 or G{alpha} only when centralspindlin was absent from the furrow. We also found spindle length or microtubule distribution did not correlate with furrow initiation. We propose that centralspindlin acts redundantly with G{alpha} to regulate furrow initiation.

Key words: Cytokinesis, Spindle midzone, Centralspindlin, Furrow initiation


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