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First published online 22 May 2007
doi: 10.1242/jcs.03461

Journal of Cell Science 120, 1967-1977 (2007)
Published by The Company of Biologists 2007
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Research Article

Nuclear localization of barrier-to-autointegration factor is correlated with progression of S phase in human cells

Tokuko Haraguchi1,2,*, Takako Koujin1, Hiroko Osakada1, Tomoko Kojidani1, Chie Mori1, Hirohisa Masuda1 and Yasushi Hiraoka1,2

1 CREST Research Project, Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe 651-2492, Japan
2 Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka 560-0043, Osaka, Japan

* Author for correspondence (e-mail: tokuko{at}nict.go.jp)

Accepted 19 April 2007

Barrier-to-autointegration factor (BAF) is a conserved metazoan protein that plays a critical role in retrovirus infection. To elucidate its role in uninfected cells, we first examined the localization of BAF in both mortal and immortal or cancerous human cell lines. In mortal cell lines (e.g. TIG-1, WI-38 and IMR-90 cells) BAF localization depended on the age of the cell, localizing primarily in the nucleus of >90% of young proliferating cells but only 20-25% of aged senescent cells. In immortal cell lines (e.g. HeLa, SiHa and HT1080 cells) BAF showed heterogeneous localization between the nucleus and cytoplasm. This heterogeneity was lost when the cells were synchronized in S phase. In S-phase-synchronized populations, the percentage of cells with predominantly nuclear BAF increased from 30% (asynchronous controls) to ~80%. In HeLa cells, RNAi-induced downregulation of BAF significantly increased the proportion of early S-phase cells that retained high levels of cyclin D3 and cyclin E expression and slowed progression through early S phase. BAF downregulation also caused lamin A to mislocalize away from the nuclear envelope. These results indicate that BAF is required for the integrity of the nuclear lamina and normal progression of S phase in human cells.

Key words: BAF, Nuclear envelope, Chromatin, Emerin, Lamin A, Aging


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T. Haraguchi, T. Kojidani, T. Koujin, T. Shimi, H. Osakada, C. Mori, A. Yamamoto, and Y. Hiraoka
Live cell imaging and electron microscopy reveal dynamic processes of BAF-directed nuclear envelope assembly
J. Cell Sci., August 1, 2008; 121(15): 2540 - 2554.
[Abstract] [Full Text] [PDF]


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