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First published online 29 May 2007
doi: 10.1242/jcs.008771

Journal of Cell Science 120, 2091-2105 (2007)
Published by The Company of Biologists 2007
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Research Article

Nucleolin functions in nucleolus formation and chromosome congression

Nan Ma, Sachihiro Matsunaga, Hideaki Takata, Rika Ono-Maniwa, Susumu Uchiyama and Kiichi Fukui*

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Osaka, Japan

* Author for correspondence (e-mail: kfukui{at}bio.eng.osaka-u.ac.jp)

Accepted 17 April 2007

A complex structure, designated the chromosome periphery, surrounds each chromosome during mitosis. Although several proteins have been shown to localize to the chromosome periphery, their functions during mitosis remain unclear. Here, we used a combination of high-resolution microscopy and RNA-interference-mediated depletion to study the functions of nucleolin, a nucleolar protein localized at the chromosome periphery, in interphase and mitosis. During mitosis, nucleolin was localized in the peripheral region including the vicinity of the outer kinetochore of chromosomes. Staining with an antibody specific for nucleolin phosphorylated by CDC2 revealed that nucleolin was also associated with the spindle poles from prometaphase to anaphase. Nucleolin depletion resulted in disorganization of the nucleoli at interphase. Furthermore, nucleolin-depleted cells showed a prolonged cell cycle with misaligned chromosomes and defects in spindle organization. The misaligned chromosomes showed syntelic kinetochore-microtubule attachments with reduced centromere stretching. Taken together, our results indicate that nucleolin is required for nucleolus formation, and is also involved in chromosome congression and spindle formation.

Key words: Nucleolin, Nucleolus formation, Chromosome congression, Mitosis


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