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First published online 5 June 2007
doi: 10.1242/jcs.003830

Journal of Cell Science 120, 2151-2161 (2007)
Published by The Company of Biologists 2007
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Research Article

Caveolin-1 is required for lateral line neuromast and notochord development

Susan J. Nixon1,2,3, Adrian Carter3, Jeremy Wegner4, Charles Ferguson1,2,3, Matthias Floetenmeyer1,2,3, Jamie Riches2, Brian Key3, Monte Westerfield4 and Robert G. Parton1,2,3,*

1 Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
2 Centre for Microscopy and Microanalysis, University of Queensland, Brisbane 4072, Australia
3 School of Biomedical Sciences, University of Queensland, Brisbane 4072, Australia
4 Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA

* Author for correspondence (e-mail: r.parton{at}imb.uq.edu.au)

Accepted 23 April 2007

Caveolae have been linked to diverse cellular functions and to many disease states. In this study we have used zebrafish to examine the role of caveolin-1 and caveolae during early embryonic development. During development, expression is apparent in a number of tissues including Kupffer's vesicle, tailbud, intersomite boundaries, heart, branchial arches, pronephric ducts and periderm. Particularly strong expression is observed in the sensory organs of the lateral line, the neuromasts and in the notochord where it overlaps with expression of caveolin-3. Morpholino-mediated downregulation of Cav1{alpha} caused a dramatic inhibition of neuromast formation. Detailed ultrastructural analysis, including electron tomography of the notochord, revealed that the central regions of the notochord has the highest density of caveolae of any embryonic tissue comparable to the highest density observed in any vertebrate tissue. In addition, Cav1{alpha} downregulation caused disruption of the notochord, an effect that was enhanced further by Cav3 knockdown. These results indicate an essential role for caveolin and caveolae in this vital structural and signalling component of the embryo.

Key words: Caveolae, Caveolin-1, Neuromast, Notochord, Zebrafish


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