spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online July 2, 2007
doi: 10.1242/10.1242/jcs.03470

Journal of Cell Science 120, 2352-2365 (2007)
Published by The Company of Biologists 2007
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ooshio, T.
Right arrow Articles by Takai, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ooshio, T.
Right arrow Articles by Takai, Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Cooperative roles of Par-3 and afadin in the formation of adherens and tight junctions

Takako Ooshio1, Naoyuki Fujita1, Akio Yamada1, Tatsuhiro Sato1, Yuichi Kitagawa1, Ryoko Okamoto1, Shinsuke Nakata1, Ayaka Miki1, Kenji Irie1,* and Yoshimi Takai1,2,{ddagger}

1 Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, Suita 565-0871, Osaka, Japan
2 Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine/Faculty of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Hyogo, Japan

{ddagger} Author for correspondence (e-mail: ytakai{at}molbio.med.osaka-u.ac.jp)

Accepted 14 May 2007

Par-3 is a cell-polarity protein that regulates the formation of tight junctions (TJs) in epithelial cells, where claudin is a major cell-cell adhesion molecule (CAM). TJs are formed at the apical side of adherens junctions (AJs), where E-cadherin and nectin are major CAMs. We have revealed that nectin first forms cell-cell adhesions, and then recruits cadherin to nectin-based cell-cell adhesion sites to form AJs and subsequently recruits claudin to the apical side of AJs to form TJs. The cytoplasmic tail of nectin binds afadin and Par-3. Afadin regulates the formation of AJs and TJs cooperatively with nectin. Here, we studied the role of Par-3 in the formation of these junctions by using Par-3-knockdown MDCK cells. Par-3 was necessary for the formation of AJs and TJs but was not necessary for nectin-based cell-cell adhesion. Par-3 promoted the association of afadin with nectin, whereas afadin was not necessary for the association of Par-3 with nectin. However, the association of afadin with nectin alone was not sufficient for the formation of AJs or TJs, and Par-3 and afadin cooperatively regulated it. We describe here these novel roles of Par-3 in the formation of junctional complexes.

Key words: Adherens junctions, Tight junctions, Nectin, Par-3, Afadin


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
M. Coureuil, G. Mikaty, F. Miller, H. Lecuyer, C. Bernard, S. Bourdoulous, G. Dumenil, R.-M. Mege, B. B. Weksler, I. A. Romero, et al.
Meningococcal Type IV Pili Recruit the Polarity Complex to Cross the Brain Endothelium
Science, July 3, 2009; 325(5936): 83 - 87.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P. R. Brakeman, K. D. Liu, K. Shimizu, Y. Takai, and K. E. Mostov
Nectin proteins are expressed at early stages of nephrogenesis and play a role in renal epithelial cell morphogenesis
Am J Physiol Renal Physiol, March 1, 2009; 296(3): F564 - F574.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
M. J. Barron, S. J. Brookes, C. E. Draper, D. Garrod, J. Kirkham, R. C. Shore, and M. J. Dixon
The cell adhesion molecule nectin-1 is critical for normal enamel formation in mice
Hum. Mol. Genet., November 15, 2008; 17(22): 3509 - 3520.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M. Sugimoto, A. Inoko, T. Shiromizu, M. Nakayama, P. Zou, S. Yonemura, Y. Hayashi, I. Izawa, M. Sasoh, Y. Uji, et al.
The keratin-binding protein Albatross regulates polarization of epithelial cells
J. Cell Biol., October 6, 2008; 183(1): 19 - 28.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. W. P. Wong, D. D. Mruk, W. M. Lee, and C. Y. Cheng
Par3/Par6 polarity complex coordinates apical ectoplasmic specialization and blood-testis barrier restructuring during spermatogenesis
PNAS, July 15, 2008; 105(28): 9657 - 9662.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
D. D. Mruk, B. Silvestrini, and C. Y. Cheng
Anchoring Junctions As Drug Targets: Role in Contraceptive Development
Pharmacol. Rev., June 1, 2008; 60(2): 146 - 180.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
H. Komura, H. Ogita, W. Ikeda, A. Mizoguchi, J. Miyoshi, and Y. Takai
Establishment of cell polarity by afadin during the formation of embryoid bodies.
Genes Cells, January 1, 2008; 13(1): 79 - 90.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2007