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First published online 17 July 2007
doi: 10.1242/jcs.007005

Journal of Cell Science 120, 2574-2585 (2007)
Published by The Company of Biologists 2007
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Research Article

T6BP and NDP52 are myosin VI binding partners with potential roles in cytokine signalling and cell adhesion

Brooke Morriswood1,*, Grigory Ryzhakov1, Claudia Puri2, Susan D. Arden2, Rhys Roberts1, Calliope Dendrou2, John Kendrick-Jones1,{ddagger} and Folma Buss2

1 MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
2 Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust/MRC Building, Hills Road, Cambridge, CB2 2XY, UK

{ddagger} Author for correspondence (e-mail: jkj{at}mrc-lmb.cam.ac.uk)

Accepted 22 May 2007

Myosin VI has been implicated in many cellular processes including endocytosis, secretion, membrane ruffling and cell motility. We carried out a yeast two-hybrid screen and identified TRAF6-binding protein (T6BP) and nuclear dot protein 52 (NDP52) as myosin VI binding partners. Myosin VI interaction with T6BP and NDP52 was confirmed in vitro and in vivo and the binding sites on each protein were accurately mapped. Immunofluorescence and electron microscopy showed that T6BP, NDP52 and myosin VI are present at the trans side of the Golgi complex, and on vesicles in the perinuclear region. Although the SKICH domain in T6BP and NDP52 does not mediate recruitment into membrane ruffles, loss of T6BP and NDP52 in RNAi knockdown cells results in reduced membrane ruffling activity and increased stress fibre and focal adhesion formation. Furthermore, we observed in these knockdown cells an upregulation of constitutive secretion of alkaline phosphatase, implying that both proteins act as negative regulators of secretory traffic at the Golgi complex. T6BP was also found to inhibit NF-{kappa}B activation, implicating it in the regulation of TRAF6-mediated cytokine signalling. Thus myosin VI-T6BP interactions may link membrane trafficking pathways with cell adhesion and cytokine-dependent cell signalling.

Key words: NDP52, T6BP, Myosin VI


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This article has been cited by other articles:


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[Abstract] [Full Text] [PDF]


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