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First published online 17 July 2007
doi: 10.1242/jcs.006346

Journal of Cell Science 120, 2683-2693 (2007)
Published by The Company of Biologists 2007
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Research Article

The neuronal Arf GAP centaurin {alpha}1 modulates dendritic differentiation

Carlene D. Moore1, Erin E. Thacker1,*, Jennifer Larimore1, David Gaston1, Alison Underwood1, Brian Kearns1,{ddagger}, Sean I. Patterson2, Trevor Jackson3, Chris Chapleau1, Lucas Pozzo-Miller1 and Anne Theibert1,§

1 Department of Neurobiology and Civitan International Research Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA
2 IHEM-CONICET, Departmento de Morfo-Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina
3 Departments of Physiology and Dermatology, School of Clinical and Laboratory Sciences, Medical School, University of Newcastle upon Tyne, NE2 4HH, UK

§ Author for correspondence (e-mail: theibert{at}nrc.uab.edu)

Accepted 24 May 2007

Centaurin {alpha}1 is an Arf GTPase-activating protein (GAP) that is highly expressed in the nervous system. In the current study, we show that endogenous centaurin {alpha}1 protein is localized in the synaptosome fraction, with peak expression in early postnatal development. In cultured dissociated hippocampal neurons, centaurin {alpha}1 localizes to dendrites, dendritic spines and the postsynaptic region. siRNA-mediated knockdown of centaurin {alpha}1 levels or overexpression of a GAP-inactive mutant of centaurin {alpha}1 leads to inhibition of dendritic branching, dendritic filopodia and spine-like protrusions in dissociated hippocampal neurons. Overexpression of wild-type centaurin {alpha}1 in cultured hippocampal neurons in early development enhances dendritic branching, and increases dendritic filopodia and lamellipodia. Both filopodia and lamellipodia have been implicated in dendritic branching and spine formation. Following synaptogenesis in cultured neurons, wild-type centaurin {alpha}1 expression increases dendritic filopodia and spine-like protrusions. Expression of a GAP-inactive mutant diminishes spine density in CA1 pyramidal neurons within cultured organotypic hippocampal slice cultures. These data support the conclusion that centaurin {alpha}1 functions through GAP-dependent Arf regulation of dendritic branching and spines that underlie normal dendritic differentiation and development.

Key words: Arf, GAP, PI 3-kinase, Dendrite, Neuronal, Development






© The Company of Biologists Ltd 2007