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First published online 24 July 2007
doi: 10.1242/jcs.009225

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The exon-junction-complex-component metastatic lymph node 51 functions in stress-granule assembly

Aurélie Baguet¹, Sébastien Degot^1,*, Nicolas Cougot², Edouard Bertrand², Marie-Pierre Chenard³, Corinne Wendling¹, Pascal Kessler¹, Hervé Le Hir⁴, Marie-Christine Rio¹ and Catherine Tomasetto^1,

¹ Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Département de Biologie du Cancer, UMR 7104 CNRS/U596 INSERM/Université Louis Pasteur, BP 10142, 67404 Illkirch, C.U. de Strasbourg, France
² Institut de Génétique Moléculaire de Montpellier, CNRS UMR 5535-IFR 24, 1919 route de Mende 34000 Montpellier, France
³ Departement de Pathologie Générale, Centre Hospitalier Universitaire de Hautepierre, 67098 Strasbourg, France
⁴ Centre de Génétique Moléculaire, CNRS UPR2167, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France

Author for correspondence (e-mail: cat{at}igbmc.u-strasbg.fr)

Accepted 11 June 2007

Metastatic lymph node 51 [MLN51 (also known as CASC3)] is a component of the exon junction complex (EJC), which is assembled on spliced mRNAs and plays important roles in post-splicing events. The four proteins of the EJC core, MLN51, MAGOH, Y14 and EIF4AIII shuttle between the cytoplasm and the nucleus. However, unlike the last three, MLN51 is mainly detected in the cytoplasm, suggesting that it plays an additional function in this compartment. In the present study, we show that MLN51 is recruited into cytoplasmic aggregates known as stress granules (SGs) together with the SG-resident proteins, fragile X mental retardation protein (FMRP), poly(A) binding protein (PABP) and poly(A)⁺ RNA. MLN51 specifically associates with SGs via its C-terminal region, which is dispensable for its incorporation in the EJC. MLN51 does not promote SG formation but its silencing, or the overexpression of a mutant lacking its C-terminal region, alters SG assembly. Finally, in human breast carcinomas, MLN51 is sometimes present in cytoplasmic foci also positive for FMRP and PABP, suggesting that SGs formation occurs in malignant tumours.

Key words: Stress granule, MLN51, Barentsz, FMRP, DCP1, Breast cancer

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