spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 24 July 2007
doi: 10.1242/jcs.009506

Journal of Cell Science 120, 2819-2827 (2007)
Published by The Company of Biologists 2007
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow All Versions of this Article:
jcs.009506v1
120/16/2819    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bannigan, A.
Right arrow Articles by Baskin, T. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bannigan, A.
Right arrow Articles by Baskin, T. I.

Research Article

A conserved role for kinesin-5 in plant mitosis

Alex Bannigan1, Wolf-Rüdiger Scheible2, Wolfgang Lukowitz3, Carey Fagerstrom1, Patricia Wadsworth1, Chris Somerville4 and Tobias I. Baskin1,*

1 Biology Department, University of Massachusetts, Amherst, MA 01003 USA
2 Max Planck Institute for Molecular Plant Physiology, Science Park Golm, 14476 Potsdam, Germany
3 Cold Spring Harbor Laboratory, 1 Bungtown Rd, Cold Spring Harbor, NY 11724, USA
4 Carnegie Institution, Department of Plant Biology, Stanford, CA 94305, USA

* Author for correspondence (e-mail: baskin{at}bio.umass.edu)

Accepted 12 June 2007

The mitotic spindle of vascular plants is assembled and maintained by processes that remain poorly explored at a molecular level. Here, we report that AtKRP125c, one of four kinesin-5 motor proteins in arabidopsis, decorates microtubules throughout the cell cycle and appears to function in both interphase and mitosis. In a temperature-sensitive mutant, interphase cortical microtubules are disorganized at the restrictive temperature and mitotic spindles are massively disrupted, consistent with a defect in the stabilization of anti-parallel microtubules in the spindle midzone, as previously described in kinesin-5 mutants from animals and yeast. AtKRP125c introduced into mammalian epithelial cells by transfection decorates microtubules throughout the cell cycle but is unable to complement the loss of the endogenous kinesin-5 motor (Eg5). These results are among the first reports of any motor with a major role in anastral spindle structure in plants and demonstrate that the conservation of kinesin-5 motor function throughout eukaryotes extends to vascular plants.

Key words: Arabidopsis thaliana, AtKRP125, Cortical microtubules, Eg5, {gamma}-tubulin, Root morphology


Related articles in JCS:

Motoring through plant mitosis

JCS 2007 120: 1601. [Full Text]  



This article has been cited by other articles:


Home page
 Plant CellHome page
M.-C. Caillaud, P. Lecomte, F. Jammes, M. Quentin, S. Pagnotta, E. Andrio, J. de Almeida Engler, N. Marfaing, P. Gounon, P. Abad, et al.
MAP65-3 Microtubule-Associated Protein Is Essential for Nematode-Induced Giant Cell Ontogenesis in Arabidopsis
PLANT CELL, February 1, 2008; 20(2): 423 - 437.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2007