spacer gif spacer gif spacer gif spacer gif spacer gif
 QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online September 19, 2007
doi: 10.1242/10.1242/jcs.009159


Journal of Cell Science 120, 3372-3383 (2007)
Published by The Company of Biologists 2007
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mathias, J. R.
Right arrow Articles by Huttenlocher, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mathias, J. R.
Right arrow Articles by Huttenlocher, A.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Live imaging of chronic inflammation caused by mutation of zebrafish Hai1

Jonathan R. Mathias1, M. Ernest Dodd1, Kevin B. Walters1, Jennifer Rhodes2, John P. Kanki2, A. Thomas Look2 and Anna Huttenlocher1,*

1 Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI 53706, USA
2 Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA 02115, USA

* Author for correspondence (e-mail: huttenlocher{at}wisc.edu)

Accepted 17 August 2007

The hallmark of chronic inflammation is the infiltration and persistence of leukocytes within inflamed tissue. Here, we describe the first zebrafish chronic inflammation mutant identified in an insertional mutagenesis screen for mutants that exhibit abnormal tissue distribution of neutrophils. We identified a mutant line with an insertion in the Hepatocyte growth factor activator inhibitor 1 gene (hai1; also known as Spint1) that showed accumulation of neutrophils in the fin. The mutant embryos exhibited inflammation in areas of epidermal hyperproliferation that was rescued by knock-down of the type II transmembrane serine protease Matriptase 1 (also known as St14), suggesting a novel role for Hai1-Matriptase 1 pathway in regulating inflammation. Using time-lapse microscopy of mutant embryos that express GFP from a neutrophil-specific promoter, we found that individual neutrophils in inflamed tissue displayed random motility characterized by periods of pausing alternating with periods of motility. During periods of persistent movement the cells were highly polarized, while the pausing modes were characterized by a loss of cell polarity. In contrast to responses to acute injury, neutrophils did not exhibit clear retrograde chemotaxis or resolution of inflammation in the mutant. These findings illustrate the utility of zebrafish as a new model system to study chronic inflammation and to visualize immune responses with high resolution in vivo.

Key words: Cell migration, Inflammation, Neutrophil, Psoriasis, Zebrafish


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in JCS:

Chronic inflammation clarified

JCS 2007 120: 1905. [Full Text]  



This article has been cited by other articles:


Home page
DevelopmentHome page
R. Szabo, J. P. Hobson, K. Christoph, P. Kosa, K. List, and T. H. Bugge
Regulation of cell surface protease matriptase by HAI2 is essential for placental development, neural tube closure and embryonic survival in mice
Development, August 1, 2009; 136(15): 2653 - 2663.
[Abstract] [Full Text] [PDF]


Home page
J BiochemHome page
Y. Miyake, M. Yasumoto, S. Tsuzuki, T. Fushiki, and K. Inouye
Activation of a Membrane-Bound Serine Protease Matriptase on the Cell Surface
J. Biochem., August 1, 2009; 146(2): 273 - 282.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Cell Physiol.Home page
J.-K. Wang, M.-S. Lee, I-C. Tseng, F.-P. Chou, Y.-W. Chen, A. Fulton, H.-S. Lee, C.-J. Chen, M. D. Johnson, and C.-Y. Lin
Polarized epithelial cells secrete matriptase as a consequence of zymogen activation and HAI-1-mediated inhibition
Am J Physiol Cell Physiol, August 1, 2009; 297(2): C459 - C470.
[Abstract] [Full Text] [PDF]


Home page
DevelopmentHome page
M. E. Dodd, J. Hatzold, J. R. Mathias, K. B. Walters, D. A. Bennin, J. Rhodes, J. P. Kanki, A. T. Look, M. Hammerschmidt, and A. Huttenlocher
The ENTH domain protein Clint1 is required for epidermal homeostasis in zebrafish
Development, August 1, 2009; 136(15): 2591 - 2600.
[Abstract] [Full Text] [PDF]


Home page
J. Leukoc. Biol.Home page
C. Hall, M. V. Flores, A. Chien, A. Davidson, K. Crosier, and P. Crosier
Transgenic zebrafish reporter lines reveal conserved Toll-like receptor signaling potential in embryonic myeloid leukocytes and adult immune cell lineages
J. Leukoc. Biol., May 1, 2009; 85(5): 751 - 765.
[Abstract] [Full Text] [PDF]


Home page
JCBHome page
S. Ausoni and S. Sartore
From fish to amphibians to mammals: in search of novel strategies to optimize cardiac regeneration
J. Cell Biol., February 9, 2009; 184(3): 357 - 364.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Pathol.Home page
K. Nagaike, M. Kawaguchi, N. Takeda, T. Fukushima, A. Sawaguchi, K. Kohama, M. Setoyama, and H. Kataoka
Defect of Hepatocyte Growth Factor Activator Inhibitor Type 1/Serine Protease Inhibitor, Kunitz Type 1 (Hai-1/Spint1) Leads to Ichthyosis-Like Condition and Abnormal Hair Development in Mice
Am. J. Pathol., November 1, 2008; 173(5): 1464 - 1475.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
R. Szabo, J. P. Hobson, K. List, A. Molinolo, C.-Y. Lin, and T. H. Bugge
Potent Inhibition and Global Co-localization Implicate the Transmembrane Kunitz-type Serine Protease Inhibitor Hepatocyte Growth Factor Activator Inhibitor-2 in the Regulation of Epithelial Matriptase Activity
J. Biol. Chem., October 24, 2008; 283(43): 29495 - 29504.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
A. Cvejic, C. Hall, M. Bak-Maier, M. V. Flores, P. Crosier, M. J. Redd, and P. Martin
Analysis of WASp function during the wound inflammatory response - live-imaging studies in zebrafish larvae
J. Cell Sci., October 1, 2008; 121(19): 3196 - 3206.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
D. Carradice and G. J. Lieschke
Zebrafish in hematology: sushi or science?
Blood, April 1, 2008; 111(7): 3331 - 3342.
[Abstract] [Full Text] [PDF]




© The Company of Biologists Ltd 2007