spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online September 19, 2007
doi: 10.1242/10.1242/jcs.002774

Journal of Cell Science 120, 3395-3403 (2007)
Published by The Company of Biologists 2007
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pu, J.
Right arrow Articles by Zhao, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pu, J.
Right arrow Articles by Zhao, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

EGF receptor signalling is essential for electric-field-directed migration of breast cancer cells

Jin Pu1, Colin D. McCaig1, Lin Cao2, Zhiqiang Zhao1, Jeffrey E. Segall3 and Min Zhao1,*,{ddagger}

1 School of Medical Sciences, University of Aberdeen, Aberdeen, AB25 2ZD, UK
2 School of Medicine, University of Aberdeen, Aberdeen, AB25 2ZD, UK
3 Department of Anatomy and Structural Biology, Program in Motility and Invasion, Albert Einstein College of Medicine, Bronx, NY 10461, USA

{ddagger} Author for correspondence (e-mail: m.zhao{at}abdn.ac.uk)

Accepted 9 July 2007

The mechanisms by which cancer cells migrate to metastasise are not fully understood. Breast cancers are accompanied by electrical depolarisation of tumour epithelial cells. The electrical changes can be detected on the skin and are used to differentiate malignant from benign breast tumours. Could the electrical signals play a role in metastasis by promoting tumour cell migration? We report that electric fields stimulate and direct migration of human breast cancer cells. Importantly, these effects were more significant in highly metastatic tumour cells than in low metastatic tumour cells. Electric-field-enhanced directional migration correlates well with the expression level of EGF receptor (EGFR/ErbB1). To confirm this, we transfected low metastatic clone MTC cells with human ErbB1, which significantly increased the electrotactic response. Inhibition of ErbB1 completely abolished the directional response of MTLn3 cells to an electric field. Transfection of MTLn3 cells and MDA-MB-435 cells with expression vectors for ErbB family members ErbB1, ErbB2 and ErbB3 also significantly enhanced EF-induced migration. These results suggest that electric signals might play a role in metastasis of breast cancers by enhancing cell migration through the ErbB-signalling pathway.

Key words: Breast cancer cells, Electric field, Migration, ErbBs, Tumour metastasis


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
C. D. McCaig, B. Song, and A. M. Rajnicek
Electrical dimensions in cell science
J. Cell Sci., December 1, 2009; 122(23): 4267 - 4276.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2007