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First published online 14 November 2007
doi: 10.1242/jcs.012468

Journal of Cell Science 120, 4093-4098 (2007)
Published by The Company of Biologists 2007
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Short Report

In vivo movement of the type V myosin Myo52 requires dimerisation but is independent of the neck domain

Agnes Grallert1, Rebeca Martín-García2, Steve Bagley3 and Daniel P. Mulvihill2,*

1 Cancer Research UK Cell Division Group, CR-UK Paterson Institute for Cancer Research, Manchester, M20 4BX, UK
2 Cell and Developmental Biology Group, Department of Biosciences, University of Kent, Canterbury, Kent, CT2 7NJ, UK
3 Advanced imaging facility, CR-UK Paterson Institute for Cancer Research, Manchester, M20 4BX, UK

* Author for correspondence (e-mail: d.p.mulvihill{at}kent.ac.uk)

Accepted 10 September 2007

Summary

Intracellular movement is a fundamental property of all cell types. Many organelles and molecules are actively transported throughout the cytoplasm by molecular motors, such as the dimeric type V myosins. These possess a long neck, which contains an IQ motif, that allow it to make 36-nm steps along the actin polymer. Live cell imaging of the fission yeast type V myosin Myo52 reveals that the protein moves rapidly throughout the cytoplasm. Here, we describe analysis of this movement and have established that Myo52 moves long distances on actin filaments in an ATP-dependent manner at ~0.5 µm/second. Myo51 and the microtubule cytoskeleton have no discernable role in modulating Myo52 movements, whereas rigour mutations in Myo52 abrogated its movement. We go on to show that, although dimerisation is required for Myo52 movement, deleting its neck has no discernable affect on Myo52 function or velocity in vivo.

Key words: Schizosaccharomyces pombe, Myosin V, IQ domains


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