spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 16 January 2007
doi: 10.1242/jcs.03347

Journal of Cell Science 120, 512-519 (2007)
Published by The Company of Biologists 2007
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.03347v1
120/3/512    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schneiderhan, W.
Right arrow Articles by Bachem, M. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schneiderhan, W.
Right arrow Articles by Bachem, M. G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Pancreatic stellate cells are an important source of MMP-2 in human pancreatic cancer and accelerate tumor progression in a murine xenograft model and CAM assay

Wilhelm Schneiderhan1,2, Fredy Diaz1, Martin Fundel1, Shaoxia Zhou1, Marco Siech3, Cornelia Hasel4, Peter Möller4, Jürgen E. Gschwend5, Thomas Seufferlein2, Thomas Gress2, Guido Adler2 and Max G. Bachem1,*

1 Department of Clinical Chemistry and Pathobiochemistry, University of Ulm, Robert-Koch-Str. 8, 89081 Ulm, Germany
2 Department of Gastroenterology, University of Ulm, Robert-Koch-Str. 8, 89081 Ulm, Germany
3 Department of Surgery, Hospital Aalen, Im Kälblesrain 1, 73430 Aalen, Germany
4 Department of Pathology, University of Ulm, Robert-Koch-Str. 8, 89081 Ulm, Germany
5 Department of Urology, University of Ulm, Prittwitzstr. 43, 89075 Ulm, Germany

* Author for correspondence (e-mail: max.bachem{at}uniklinik-ulm.de)

Accepted 15 November 2006

The effect of the characteristic desmoplastic reaction of pancreatic cancer on tumor progression is largely unknown. We investigated whether pancreatic stellate cells, which are responsible for the desmoplastic reaction, support tumor progression. Immunohistology revealed that matrix metalloproteinase-2 (MMP-2), which is suggested to promote pancreatic cancer progression, is present in stellate cells adjacent to cancer cells. In vitro, stellate cells exhibited a much higher basal expression of MMP-2 compared with cancer cells. Panc1-, MiaPaCa2- and SW850-conditioned media stimulated MMP-2 release of stellate cells as detected by zymography. Cancer cells expressed and released basigin [BSG, extracellular matrix metalloproteinase inducer (EMMPRIN), CD147], a glycoprotein that is known to stimulate MMP-2 in mesenchymal cells, as detected by immunostaining, western blot and reverse transcription-polymerase chain reaction. Tumor cell-conditioned medium and BSG purified by affinity chromatography from supernatants of cancer cells, but not supernatants depleted from BSG, stimulated expression of MMP-1 and MMP-2 of stellate cells as demonstrated by western blot and zymography. Moreover, the interaction of stellate cells and cancer cells promoted the invasiveness of Panc-1 cells in the chorioallantoic membrane assay and increased the weight of tumors induced by all carcinoma cell lines in nude mice by 2.1-3.7-fold. Our findings support the assumption that the interaction of stellate cells and cancer cells promotes progression of pancreatic cancer.

Key words: Pancreatic adenocarcinoma, Tumor-host interaction, Matrix metalloproteinases, Pancreatic stellate cell, Mouse xenograft model


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in JCS:

The stars of pancreatic cancer

JCS 2007 120: 305. [Full Text]  



This article has been cited by other articles:


Home page
 GutHome page
W Schneiderhan, M Scheler, K-H Holzmann, M Marx, J E Gschwend, M Bucholz, T M Gress, T Seufferlein, G Adler, and F Oswald
CD147 silencing inhibits lactate transport and reduces malignant potential of pancreatic cancer cells in in vivo and in vitro models
Gut, October 1, 2009; 58(10): 1391 - 1398.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
F. E.M. Froeling, T. A. Mirza, R. M. Feakins, A. Seedhar, G. Elia, I. R. Hart, and H. M. Kocher
Organotypic Culture Model of Pancreatic Cancer Demonstrates that Stromal Cells Modulate E-Cadherin, {beta}-Catenin, and Ezrin Expression in Tumor Cells
Am. J. Pathol., August 1, 2009; 175(2): 636 - 648.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. F. Hwang, T. Moore, T. Arumugam, V. Ramachandran, K. D. Amos, A. Rivera, B. Ji, D. B. Evans, and C. D. Logsdon
Cancer-Associated Stromal Fibroblasts Promote Pancreatic Tumor Progression
Cancer Res., February 1, 2008; 68(3): 918 - 926.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2007