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First published online 27 February 2007
doi: 10.1242/jcs.03404

Journal of Cell Science 120, 1061-1071 (2007)
Published by The Company of Biologists 2007
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Research Article

Integrin-ECM interactions regulate the changes in cell shape driving the morphogenesis of the Drosophila wing epithelium

Paloma Domínguez-Giménez1, Nicholas H. Brown2 and María D. Martín-Bermudo3,*

1 Institut de Reserca Biomédica, Barcelona 08028, Spain
2 The Gurdon Institute and Dept. of PDN, University of Cambridge, Cambridge, CB21QR, UK
3 Centro Andaluz de Biología del Desarrollo (CSIC), Universidad Pablo de Olavide, Sevilla 41013, Spain

* Author for correspondence (e-mail: mdmarber{at}upo.es)

Accepted 11 January 2007

During development, morphogenesis involves migration and changes in the shape of epithelial sheets, both of which require coordination of cell adhesion. Thus, while modulation of integrin-mediated adhesion to the ECM regulates epithelial motility, cell-cell adhesion via cadherins controls the remodelling of epithelial sheets. We have used the Drosophila wing epithelium to demonstrate that cell-ECM interactions mediated by integrins also regulate the changes in cell shape that underly epithelial morphogenesis. We show that integrins control the transitions from columnar to cuboidal cell shape underlying wing formation, and we demonstrate that eliminating the ECM has the same effect on cell shape as inhibiting integrin function. Furthermore, lack of integrin activity also induces detachment of the basal lamina and failure to assemble the basal matrix. Hence, we propose that integrins control epithelial cell shape by mediating adherence of these cells to the ECM. Finally, we show that the ECM has an instructive rather than a structural role, because inhibition of Raf reverses the cell shape changes caused by perturbing integrins.

Key words: Cell-ECM adhesion, Integrins, Cell shape, Epithelial morphogenesis, Raf




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© The Company of Biologists Ltd 2007