spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 13 March 2007
doi: 10.1242/jcs.03420

Journal of Cell Science 120, 1209-1215 (2007)
Published by The Company of Biologists 2007
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow All Versions of this Article:
jcs.03420v1
120/7/1209    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bidla, G.
Right arrow Articles by Theopold, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bidla, G.
Right arrow Articles by Theopold, U.

Research Article

Crystal cell rupture after injury in Drosophila requires the JNK pathway, small GTPases and the TNF homolog Eiger

Gawa Bidla1, Mitchell S. Dushay2 and Ulrich Theopold1,*

1 Department of Molecular Biology and Functional Genomics, University of Stockholm, Svante Arrheniusväg 16-18, SE 10691 Stockholm, Sweden
2 Department of Comparative Physiology, Uppsala University, Norbyvägen 18A, SE 75236 Uppsala, Sweden

* Author for correspondence (e-mail: uli{at}molbio.su.se)

Accepted 1 February 2007

The prophenoloxidase-activating cascade is a key component of arthropod immunity. Drosophila prophenoloxidase is stored in crystal cells, a specialized class of blood cells from which it is released through cell rupture. Within minutes after bleeding, prophenoloxidase is activated leading to visible melanization of the clot matrix. Using crystal cell rupture and melanization as readouts to screen mutants in signal transduction pathways, we show that prophenoloxidase release requires Jun N-terminal kinase, small Rho GTPases and Eiger, the Drosophila homolog of tumor necrosis factor. We also provide evidence that in addition to microbial products, endogenous signals from dying hemocytes contribute to triggering and/or assembly of the prophenoloxidase-activating cascade, and that this process can be inhibited in vitro and in vivo using the viral apoptotic inhibitor p35. Our results provide a more comprehensive view of immune signal transduction pathways, with implications for immune reactions where cell death is used as a terminal mode of cell activation.

Key words: Innate immunity, Phenoloxidase, Apoptosis, Hemocytes, JNK, TNF




This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
M. M. Davis, D. A. Primrose, and R. B. Hodgetts
A Member of the p38 Mitogen-Activated Protein Kinase Family Is Responsible for Transcriptional Induction of Dopa decarboxylase in the Epidermis of Drosophila melanogaster during the Innate Immune Response
Mol. Cell. Biol., August 1, 2008; 28(15): 4883 - 4895.
[Abstract] [Full Text] [PDF]

Home page
 DMMHome page
M. S. Dionne and D. S. Schneider
Models of infectious diseases in the fruit fly Drosophila melanogaster
Disease Models and Mechanisms, July 1, 2008; 1(1): 43 - 49.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
A. Avet-Rochex, J. Perrin, E. Bergeret, and M.-O. Fauvarque
Rac2 is a major actor of Drosophila resistance to Pseudomonas aeruginosa acting in phagocytic cells
Genes Cells, October 1, 2007; 12(10): 1193 - 1204.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2007