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First published online 13 March 2007
doi: 10.1242/jcs.03425

Journal of Cell Science 120, 1245-1255 (2007)
Published by The Company of Biologists 2007
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Research Article

Aurora B controls the association of condensin I but not condensin II with mitotic chromosomes

Jesse J. Lipp1,2,*, Toru Hirota1,{ddagger}, Ina Poser2 and Jan-Michael Peters1,§

1 Research Institute of Molecular Pathology, Dr Bohrgasse 7, A-1030 Vienna, Austria
2 Max Planck Institute of Molecular Cell Biology and Genetics Dresden, Pfotenhauerstr. 108, D-01307 Dresden, Germany

§ Author for correspondence (e-mail: peters{at}imp.univie.ac.at)

Accepted 1 February 2007

The assembly of mitotic chromosomes is controlled by condensin complexes. In vertebrates, condensin I binds to chromatin in prometaphase, confers rigidity to chromosomes and enables the release of cohesin complexes from chromosome arms, whereas condensin II associates with chromosomes in prophase and promotes their condensation. Both complexes are essential for chromosome segregation in anaphase. Although the association of condensins with chromatin is important for the assembly and segregation of mitotic chromosomes, it is poorly understood how this process is controlled. Here we show that the mitotic kinase Aurora B regulates the association of condensin I, but not the interaction of condensin II with chromatin. Quantitative time-lapse imaging of cells expressing GFP-tagged condensin subunits revealed that Aurora B is required for efficient loading of condensin I onto chromosomes in prometaphase and for maintenance of the complex on chromosomes in later stages of mitosis. The three non-SMC subunits of condensin I are Aurora B substrates in vitro and their mitosis-specific phosphorylation depends on Aurora B in vivo. Our data indicate that Aurora B contributes to chromosome rigidity and segregation by promoting the binding of condensin I to chromatin. We have also addressed how Aurora B might mediate the dissociation of cohesin from chromosome arms.

Key words: Mitosis, Chromosome condensation, Aurora B, Condensin, Sgo1


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