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First published online 20 March 2007
doi: 10.1242/jcs.006924

Journal of Cell Science 120, 1325-1329 (2007)
Published by The Company of Biologists 2007
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Short Report

The AAA-ATPase p97-Ufd1-Npl4 is required for ERAD but not for spindle disassembly in Xenopus egg extracts

Simone Heubes and Olaf Stemmann*

Department of Molecular Cell Biology, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany

* Author for correspondence (e-mail: stemmann{at}biochem.mpg.de)

Accepted 14 February 2007

Summary

The highly abundant AAA-ATPase p97 is required for diverse cellular processes, of which ER-associated protein degradation (ERAD) is understood best. Previously, a new role of p97 in spindle disassembly at the end of mitosis has been reported. However, we show that neither addition of dominant-negative p97 mutants nor depletion of crucial p97 adaptors impairs transition of meiotic spindles into interphase arrays of microtubules. The dominant-negative approach is validated by inhibition of ERAD, which we reconstitute for the first time in the powerful biochemical system of Xenopus egg extracts. The role of p97 in spindle disassembly during meiotic exit should therefore be reconsidered.

Key words: Microtubules, Mitotic exit, p97, Spindle disassembly, Xenopus egg extracts


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This article has been cited by other articles:


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[Abstract] [Full Text] [PDF]


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