|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online 27 March 2007
doi: 10.1242/jcs.000968
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA
* Author for correspondence (e-mail: john.eppig{at}jax.org)
Accepted 13 February 2007
The two principal functions of ovarian follicles are developmental and endocrine. The cumulus cells surrounding the oocyte are specialized to serve the development of the oocyte and steroidogenesis is a principal role of mural granulosa cells that line the follicle wall. The findings in this report demonstrate that oocytectomy or treatment with an inhibitor of SMAD2/3 activation results in decreased cumulus marker mRNA transcript levels and allows FSH to induce mural marker transcripts in cumulus cells. In addition, SMAD2/3 signaling is involved in enabling cumulus expansion and EGF-induced increases in Ptx3, Ptgs2 and Has2 mRNA levels. By contrast, follicle-stimulating hormone (FSH) stimulated expression of mural transcripts, but suppressed levels of cumulus transcripts. Thus, FSH and oocyte-stimulated SMAD2/3 signaling establish opposing gradients of influence in the follicle. These specify the mural and cumulus granulosa cell phenotypes that are pivotal for appropriate endocrine function and oocyte development.
Key words: Ovarian follicle, Oocytes, FSH, SMAD2/3
Related articles in JCS:
This article has been cited by other articles:
|
|
 |
|
  A Martelli, N Bernabo, P Berardinelli, V Russo, C Rinaldi, O Di Giacinto, A Mauro, and B Barboni Vascular supply as a discriminating factor for pig preantral follicle selection Reproduction, January 1, 2009; 137(1): 45 - 58. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Li, S. A. Pangas, C. J. Jorgez, J. M. Graff, M. Weinstein, and M. M. Matzuk Redundant Roles of SMAD2 and SMAD3 in Ovarian Granulosa Cells In Vivo Mol. Cell. Biol., December 1, 2008; 28(23): 7001 - 7011. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Li, L. J. McKenzie, and M. M. Matzuk Revisiting oocyte-somatic cell interactions: in search of novel intrafollicular predictors and regulators of oocyte developmental competence Mol. Hum. Reprod., December 1, 2008; 14(12): 673 - 678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Swain and T. B. Pool ART failure: oocyte contributions to unsuccessful fertilization Hum. Reprod. Update, September 1, 2008; 14(5): 431 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ning, H. Ouyang, S. Wang, X. Chen, B. Xu, J. Yang, H. Zhang, M. Zhang, and G. Xia 3',5'-Cyclic Adenosine Monophosphate Response Element Binding Protein Up-Regulated Cytochrome P450 Lanosterol 14{alpha}-Demethylase Expression Involved in Follicle-Stimulating Hormone-Induced Mouse Oocyte Maturation Mol. Endocrinol., July 1, 2008; 22(7): 1682 - 1694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Gilchrist, M. Lane, and J. G. Thompson Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality Hum. Reprod. Update, March 1, 2008; 14(2): 159 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-Q. Su, K. Sugiura, K. Wigglesworth, M. J. O'Brien, J. P. Affourtit, S. A. Pangas, M. M. Matzuk, and J. J. Eppig Oocyte regulation of metabolic cooperativity between mouse cumulus cells and oocytes: BMP15 and GDF9 control cholesterol biosynthesis in cumulus cells Development, January 1, 2008; 135(1): 111 - 121. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. J Diaz, K. Sugiura, and J. J Eppig Regulation of Pcsk6 Expression During the Preantral to Antral Follicle Transition in Mice: Opposing Roles of FSH and Oocytes Biol Reprod, January 1, 2008; 78(1): 176 - 183. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Feuerstein, V. Cadoret, R. Dalbies-Tran, F. Guerif, R. Bidault, and D. Royere Gene expression in human cumulus cells: one approach to oocyte competence Hum. Reprod., December 1, 2007; 22(12): 3069 - 3077. [Abstract] [Full Text] [PDF]
|
|
