spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 4 December 2007
doi: 10.1242/jcs.019414

Journal of Cell Science 121, 65-74 (2008)
Published by The Company of Biologists 2008
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow All Versions of this Article:
jcs.019414v1
121/1/65    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gogendeau, D.
Right arrow Articles by Beisson, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gogendeau, D.
Right arrow Articles by Beisson, J.

Research Article

Functional diversification of centrins and cell morphological complexity

Delphine Gogendeau1,2,3,*, Catherine Klotz1,2,3, Olivier Arnaiz1,2,3, Agata Malinowska4, Michal Dadlez4,5, Nicole Garreau de Loubresse1,2,3, Françoise Ruiz1,2,3, France Koll1,2,3 and Janine Beisson1,2,3

1 CNRS, Centre de Génétique Moléculaire, UPR 2167, Gif-sur-Yvette, F-91198, France
2 Université Paris-Sud, Orsay, Paris, F-91405, France
3 Université Pierre et Marie Curie, Paris 6, F-75005, France
4 Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw Pawinskiego 5a, Poland
5 Department of Biology, University of Warsaw, ul. Miecznikowa 3, 02-091 Warsaw, Poland

* Author for correspondence (e-mail: gogendeau{at}cgm.cnrs-gif.fr)

Accepted 9 October 2007

In addition to their key role in the duplication of microtubule organising centres (MTOCs), centrins are major constituents of diverse MTOC-associated contractile arrays. A centrin partner, Sfi1p, has been characterised in yeast as a large protein carrying multiple centrin-binding sites, suggesting a model for centrin-mediated Ca2+-induced contractility and for the duplication of MTOCs. In vivo validation of this model has been obtained in Paramecium, which possesses an extended contractile array – the infraciliary lattice (ICL) – essentially composed of centrins and a huge Sfi1p-like protein, PtCenBP1p, which is essential for ICL assembly and contractility. The high molecular diversity revealed here by the proteomic analysis of the ICL, including ten subfamilies of centrins and two subfamilies of Sf1p-like proteins, led us to address the question of the functional redundancy, either between the centrin-binding proteins or between the centrin subfamilies. We show that all are essential for ICL biogenesis. The two centrin-binding protein subfamilies and nine of the centrin subfamilies are ICL specific and play a role in its molecular and supramolecular architecture. The tenth and most conserved centrin subfamily is present at three cortical locations (ICL, basal bodies and contractile vacuole pores) and might play a role in coordinating duplication and positioning of cortical organelles.

Key words: Basal bodies, Centrin, Centrin-binding proteins, Cytoskeleton organisation, Paramecium




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
J. Shi, J. B. Franklin, J. T. Yelinek, I. Ebersberger, G. Warren, and C. Y. He
Centrin4 coordinates cell and nuclear division in T. brucei
J. Cell Sci., September 15, 2008; 121(18): 3062 - 3070.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2008