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First published online 29 April 2008
doi: 10.1242/jcs.026344

Journal of Cell Science 121, 1633-1640 (2008)
Published by The Company of Biologists 2008
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Research Article

Dnm1p-dependent peroxisome fission requires Caf4p, Mdv1p and Fis1p

Alison M. Motley, Gemma P. Ward and Ewald H. Hettema*

Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK

* Author for correspondence (e-mail: e.hettema{at}sheffield.ac.uk)

Accepted 21 February 2008

Yeast peroxisomes multiply by fission. Fission requires two dynamin-related proteins, Dnm1p and Vps1p. Using an in vivo fission assay, we show that Dnm1p-dependent peroxisome fission requires Fis1p, Caf4p and Mdv1p. Fluorescence microscopy of cells expressing GFP-tagged Caf4p and Mdv1p revealed that their association with peroxisomes relies on Fis1p. Vps1p-dependent peroxisome fission occurs independently of these factors. Vps1p contributes most to fission of peroxisomes when cells are grown on glucose. Overexpression of Dnm1p suppresses the fission defect as long as Fis1p and either Mdv1p or Caf4p are present. Conversely, overexpression of Dnm1p does not restore the vacuolar fusion defect of vps1 cells and Vps1p overexpression does not restore the mitochondrial fission defect of dnm1 cells. These data show that Vps1p and Dnm1p are part of independent fission machineries. Because the contribution of Dnm1p to peroxisome fission appears to be more pronounced in cells that proliferate peroxisomes in response to mitochondrial dysfunction, Dnm1p might be part of the mechanism that coordinates mitochondrial and peroxisomal biogenesis.

Key words: Caf4, Mdv1, Dnm1, Peroxisome


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[Abstract] [Full Text] [PDF]


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