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First published online 29 April 2008
doi: 10.1242/jcs.022640

Journal of Cell Science 121, 1681-1692 (2008)
Published by The Company of Biologists 2008
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Research Article

Isoform B of myosin II heavy chain mediates actomyosin contractility during TNF{alpha}-induced apoptosis

Sara Solinet and María Leiza Vitale*

Department of Pathology and Cell Biology, Université de Montréal, 2900 Edouard-Montpetit, Montréal, Québec, H3T 1J4, Canada

* Author for correspondence (e-mail: maria.leiza.vitale{at}umontreal.ca)

Accepted 19 February 2008

Cells that are treated long-term with TNF{alpha} or short-term with TGF{alpha} together with cycloheximide (CHX) undergo apoptosis. Cell shrinkage and detachment during apoptosis is dependent on actomyosin contractility. Myosin II heavy chain (MHCII) isoforms have shared and distinct functions. Here, we investigated whether the involvement of MHCII isoforms A and B (MHCIIA and MHCIIB, respectively) in cell shrinkage and detachment differs during apoptosis. We show that TNF{alpha} induces caspase-dependent MHCIIA degradation, whereas MHCIIB levels and association with the cytoskeleton remained virtually unchanged in TtT/GF cells and NIH 3T3 fibroblasts. MHCIIA proteolysis also occurred in fibroblasts that lack MHCIIB when treated with TNF{alpha} and CHX together. The absence of MHCIIB did not affect cell death rate. However, MHCIIB–/– cells showed more resistance to TNF{alpha}–induced actin disassembly, cell shrinkage and detachment than wild-type fibroblasts, indicating the participation of MHCIIB in these events. Moreover, inhibition of atypical PKC{zeta}, which targets MHCIIB but not MHCIIA, blocked TNF{alpha}-induced shrinkage and detachment in TtT/GF cells and wild-type fibroblasts, but the inhibitory effect was significantly reduced in MHCIIB–/– fibroblasts. TNF{alpha} treatment increased cytoskeleton-associated myosin light chain (MLC) phosphorylation but did not induce actin cleavage. In conclusion, our results demonstrate that MHCIIB, together with MLC phosphorylation and actin, constitute the actomyosin cytoskeleton that mediates contractility during apoptosis.

Key words: Myosin, Apoptosis, Actin


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© The Company of Biologists Ltd 2008