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First published online 29 April 2008
doi: 10.1242/jcs.019174

Journal of Cell Science 121, 1718-1726 (2008)
Published by The Company of Biologists 2008
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Research Article

EML3 is a nuclear microtubule-binding protein required for the correct alignment of chromosomes in metaphase

Justus Tegha-Dunghu1, Beate Neumann2, Simone Reber1, Roland Krause3, Holger Erfle2, Thomas Walter2, Michael Held2, Phill Rogers2, Kerstin Hupfeld1, Thomas Ruppert1, Jan Ellenberg2 and Oliver J. Gruss1,*

1 Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
2 MitoCheck Project Group, European Molecular Biology Laboratory, Meyerhofstr.1, 69117 Heidelberg, Germany
3 Max-Planck-Institut für Molekulare Genetik, Ihnestr. 73, 14195 Berlin, Germany

* Author for correspondence (e-mail: o.gruss{at}zmbh.uni-heidelberg.de)

Accepted 28 February 2008

Assembly of the mitotic spindle requires a global change in the activity and constitution of the microtubule-binding-protein array at mitotic onset. An important subset of mitotic microtubule-binding proteins localises to the nucleus in interphase and essentially contributes to spindle formation and function after nuclear envelope breakdown. Here, we used a proteomic approach to selectively identify proteins of this category and revealed 50 poorly characterised human gene products, among them the echinoderm microtubule-associated-protein-like gene product, EML3. Indirect immunofluorescence showed that EML3 colocalises with spindle microtubules throughout all mitotic stages. In interphase, EML3 colocalised with cytoplasmic microtubules and accumulated in interphase nuclei. Using YFP-fusion constructs of EML3, we located a nuclear localisation signal and confirmed the microtubule-binding domain of EML3. Functional analysis of EML3 using time-lapse fluorescence microscopy and detailed end-point analysis of phenotypes after siRNA knockdown demonstrates an important role for EML3 in correct metaphase chromosome alignment. Our proteomic identification screen combined with sensitive phenotypic analysis therefore provides a reliable platform for the identification and characterisation of proteins important for correct cell division.

Key words: Spindle proteins, Nuclear Proteins, EML3


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