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First published online 13 May 2008
doi: 10.1242/jcs.027334

Journal of Cell Science 121, 1869-1875 (2008)
Published by The Company of Biologists 2008
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Research Article

Reduction of Crk and CrkL expression blocks reelin-induced dendritogenesis

Tohru Matsuki, Albéna Pramatarova and Brian W. Howell*

Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, NIH, 35 Convent Drive, Bethesda, MD 20892, USA

* Author for correspondence (e-mail: howellb{at}ninds.nih.gov)

Accepted 17 March 2008

The reelin signaling pathway regulates nervous system function after birth, in addition to its role in regulating neuronal positioning during embryogenesis. The receptor-dependent, reelin-induced tyrosine phosphorylation of the Dab1 docking protein is an established prerequisite for biological responses to this ligand. Here we show that the inactivation of a conditional Dab1 allele reduces process complexity in correctly positioned neurons in the CA1 region of the mouse hippocampus after birth. Reelin stimulation of cultured hippocampal neurons enhances dendritogenesis by approximately twofold and in a manner dependent on Src family kinases. This enhancement is blocked by reducing expression of Crk family proteins, adaptor molecules that interact with Dab1 in a tyrosine phosphorylation-dependent manner. Retrovirally expressed inhibitory RNAs used to reduce Crk and CrkL expression did not block BDNF-enhanced dendritogenesis or influence axonogenesis. Together, this demonstrates that the Crk family proteins are important downstream components of the reelin signaling pathway in the regulation of postnatal hippocampal dendritogenesis.

Key words: Dab1, Crk, CrkL, Dendritogenesis, Hippocampus


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T. Matsuki, A. Pramatarova, and B. W. Howell
Reduction of Crk and CrkL expression blocks reelin-induced dendritogenesis
Development, June 15, 2008; 135(12): e1 - e1.
[Full Text]


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