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First published online 13 May 2008
doi: 10.1242/jcs.026989

Journal of Cell Science 121, 1876-1886 (2008)
Published by The Company of Biologists 2008
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Research Article

Replication-timing-correlated spatial chromatin arrangements in cancer and in primate interphase nuclei

Florian Grasser1, Michaela Neusser1, Heike Fiegler2, Tobias Thormeyer1, Marion Cremer1, Nigel P. Carter2, Thomas Cremer1,3 and Stefan Müller1,*

1 Department of Biology II, Human Genetics, Ludwig-Maximilians University Munich, 82152 Planegg-Martinsreid, Germany
2 The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge CB10 1SA, UK
3 Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians University Munich, Munich, Germany

* Author for correspondence (e-mail: s.mueller{at}lrz.uni-muenchen.de)

Accepted 17 March 2008

Using published high-resolution data on S-phase replication timing, we determined the three-dimensional (3D) nuclear arrangement of 33 very-early-replicating and 31 very-late-replicating loci. We analyzed diploid human, non-human primate and rearranged tumor cells by 3D fluorescence in situ hybridization with the aim of investigating the impact of chromosomal structural changes on the nuclear organization of these loci. Overall, their topology was found to be largely conserved between cell types, species and in tumor cells. Early-replicating loci were localized in the nuclear interior, whereas late-replicating loci showed a broader distribution with a higher preference for the periphery than for late-BrdU-incorporation foci. However, differences in the spatial arrangement of early and late loci of chromosome 2, as compared with those from chromosome 5, 7 and 17, argue against replication timing as a major driving force for the 3D radial genome organization in human lymphoblastoid cell nuclei. Instead, genomic properties, and local gene density in particular, were identified as the decisive parameters. Further detailed comparisons of chromosome 7 loci in primate and tumor cells suggest that the inversions analyzed influence nuclear topology to a greater extent than the translocations, thus pointing to geometrical constraints in the 3D conformation of a chromosome territory.

Key words: Nuclear architecture, Replication timing, Chromosome territory, Tumor, Primate


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