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First published online 24 June 2008
doi: 10.1242/jcs.025452

Journal of Cell Science 121, 2415-2422 (2008)
Published by The Company of Biologists 2008
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Research Article

Lipid droplets are arrested in the ER membrane by tight binding of lipidated apolipoprotein B-100

Yuki Ohsaki, Jinglei Cheng, Michitaka Suzuki, Akikazu Fujita and Toyoshi Fujimoto*

Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan

* Author for correspondence (e-mail: tfujimot{at}med.nagoya-u.ac.jp)

Accepted 22 April 2008

Apolipoprotein B-100 (ApoB) is a major component of very-low-density lipoproteins, and is deposited in a region around lipid droplets (LDs) called the `ApoB-crescent'. The ApoB-crescent is thought to be related to ApoB degradation because it drastically increases when proteasome or autophagy is inhibited. In the present study, we found that ApoB-crescents were significantly reduced when ApoB lipidation was suppressed by either the inhibition or knockdown of the microsomal triglyceride-transfer protein. By contrast, ApoB-crescents increased under conditions that are presumed to cause lipidated ApoB abnormalities in secretory compartments. By electron microscopic analyses, we identified the ApoB-crescent as a thin cholesterol-rich ER cistern fused to an LD, and – topologically – this structure is equivalent to a lipid-ester globule between the two leaflets of the ER membrane. ApoB localized in the thin cisternal lumen, and its binding to LDs was resistant to alkaline treatment. Overexpression of ADRP or TIP47 suppressed the increase in the number of ApoB-crescents, whereas knockdown of these proteins had the opposite effect. From these results, we inferred that the ApoB-crescent is formed by an LD that is arrested in the ER membrane by tight binding of lipidated ApoB to its luminal surface. We suggest that ApoB processing and LD formation are closely linked.

Key words: Lipid droplet, ER, Apolipoprotein B, Microsomal triglyceride transfer protein (MTP), Adipose differentiation-related protein (ADFP, ADRP), TIP47


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