spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 24 July 2008
doi: 10.1242/jcs.027599

Journal of Cell Science 121, 2629-2634 (2008)
Published by The Company of Biologists 2008
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow All Versions of this Article:
jcs.027599v1
121/16/2629    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Parisi, S.
Right arrow Articles by Russo, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Parisi, S.
Right arrow Articles by Russo, T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Short Report

Klf5 is involved in self-renewal of mouse embryonic stem cells

Silvia Parisi1,2,*, Fabiana Passaro1,3,*, Luigi Aloia1,2, Ichiro Manabe4, Ryozo Nagai5, Lucio Pastore1,3 and Tommaso Russo1,3,{ddagger}

1 CEINGE Biotecnologie Avanzate, 80145 Napoli, Italy
2 European School of Molecular Medicine, SEMM, 80145 Napoli, Italy
3 Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, 80131 Napoli, Italy
4 Nano-Bioengineering Education Program, The University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo, Tokyo 113-8655, Japan
5 Department of Cardiovascular Medicine, The University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo, Tokyo 113-8655, Japan

{ddagger} Author for correspondence (e-mail: russot{at}dbbm.unina.it)

Accepted 15 May 2008

Summary

Self-renewal of embryonic stem cells (ESCs) is maintained by a complex regulatory mechanism involving transcription factors Oct3/4 (Pou5f1), Nanog and Sox2. Here, we report that Klf5, a Zn-finger transcription factor of the Kruppel-like family, is involved in ESC self-renewal. Klf5 is expressed in mouse ESCs, blastocysts and primordial germ cells, and its knockdown by RNA interference alters the molecular phenotype of ESCs, thereby preventing their correct differentiation. The ability of Klf5 to maintain ESCs in the undifferentiated state is supported by the finding that differentiation of ESCs is prevented when Klf5 is constitutively expressed. Maintenance of the undifferentiated state by Klf5 is, at least in part, due to the control of Nanog and Oct3/4 transcription, because Klf5 directly binds to the promoters of these genes and regulates their transcription.

Key words: Differentiation, Kruppel-like factors, Nanog, Oct3/4, Self-renewal


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
P. Guo, X.-Y. Dong, K. Zhao, X. Sun, Q. Li, and J.-T. Dong
Opposing Effects of KLF5 on the Transcription of MYC in Epithelial Proliferation in the Context of Transforming Growth Factor {beta}
J. Biol. Chem., October 9, 2009; 284(41): 28243 - 28252.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
I. Chambers and S. R. Tomlinson
The transcriptional foundation of pluripotency
Development, July 15, 2009; 136(14): 2311 - 2322.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2008