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First published online 24 July 2008
doi: 10.1242/jcs.027144

Journal of Cell Science 121, 2643-2651 (2008)
Published by The Company of Biologists 2008
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Research Article

Dynein and Star interact in EGFR signaling and ligand trafficking

Stanley J. P. Iyadurai1, John T. Robinson1, Lingzhi Ma1, Yungui He1, Sarah Mische1, Min-gang Li1, William Brown1, Annabel Guichard2, Ethan Bier2 and Thomas S. Hays1,*

1 University of Minnesota, Department of Genetics, Cell Biology and Development, Minneapolis, MN 55455, USA
2 Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093, USA

* Author for correspondence (e-mail: haysx001{at}umn.edu)

Accepted 26 May 2008

Intracellular transport and processing of ligands is critical to the activation of signal transduction pathways that guide development. Star is an essential gene in Drosophila that has been implicated in the trafficking of ligands for epidermal growth factor (EGF) receptor signaling. The role of cytoplasmic motors in the endocytic and secretory pathways is well known, but the specific requirement of motors in EGF receptor transport has not been investigated. We identified Star in a screen designed to recover second-site modifiers of the dominant rough eye phenotype of the Glued mutation Gl1. The Glued (Gl) locus encodes the p150 subunit of the dynactin complex, an activator of cytoplasmic dynein-driven motility. We show that alleles of Gl and dynein genetically interact with both Star and EGFR alleles. Similarly to mutations in Star, the Gl1 mutation is capable of modifying the phenotypes of the EGFR mutation Ellipse. These genetic interactions suggest a model in which Star, dynactin and dynein cooperate in the trafficking of EGF ligands. In support of this model, overexpression of the cleaved, active Spitz ligand can partially bypass defective trafficking and suppress the genetic interactions. Our direct observations of live S2 cells show that export of Spitz-GFP from the endoplasmic reticulum, as well as the trafficking of Spitz-GFP vesicles, depends on both Star and dynein.

Key words: Star, Dynein, Spitz, Drosophila


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Dynein and Star interact in EGFR signaling and ligand trafficking
Development, September 1, 2008; 135(17): e1 - e1.
[Full Text]


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