spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online September 3, 2008
doi: 10.1242/10.1242/jcs.032649

Journal of Cell Science 121, 3083-3091 (2008)
Published by The Company of Biologists 2008
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, H.
Right arrow Articles by Geller, H. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, H.
Right arrow Articles by Geller, H. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Chondroitin-4-sulfation negatively regulates axonal guidance and growth

Hang Wang1,*, Yasuhiro Katagiri1,*,{ddagger}, Thomas E. McCann1, Edward Unsworth2, Paul Goldsmith2, Zu-Xi Yu3, Fei Tan1, Lizzie Santiago1, Edward M. Mills4, Yu Wang5, Aviva J. Symes5 and Herbert M. Geller1

1 Developmental Neurobiology Section, National Institutes of Health, Bethesda, MD 20892, USA
3 Pathology Core, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
2 Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
4 Division of Pharmacology/Toxicology, College of Pharmacy, The University of Texas at Austin, Austin, TX 78712, USA
5 Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA

{ddagger} Author for correspondence (e-mail: katagir{at}helix.nih.gov)

Accepted 25 June 2008

Glycosaminoglycan (GAG) side chains endow extracellular matrix proteoglycans with diversity and complexity based upon the length, composition and charge distribution of the polysaccharide chain. Using cultured primary neurons, we show that specific sulfation in the GAG chains of chondroitin sulfate mediates neuronal guidance cues and axonal growth inhibition. Chondroitin-4-sulfate (CS-A), but not chondroitin-6-sulfate (CS-C), exhibits a strong negative guidance cue to mouse cerebellar granule neurons. Enzymatic and gene-based manipulations of 4-sulfation in the GAG side chains alter their ability to direct growing axons. Furthermore, 4-sulfated chondroitin sulfate GAG chains are rapidly and significantly increased in regions that do not support axonal regeneration proximal to spinal cord lesions in mice. Thus, our findings show that specific sulfation along the carbohydrate backbone carries instructions to regulate neuronal function.

Key words: Chondroitin sulfate proteoglycan, Sulfation, Axonal guidance, Axonal growth


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in JCS:

CSPGs show axons the way

JCS 2008 121: 1802. [Full Text]  





© The Company of Biologists Ltd 2008