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First published online 16 September 2008
doi: 10.1242/jcs.028134

Journal of Cell Science 121, 3347-3356 (2008)
Published by The Company of Biologists 2008
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Research Article

{alpha}II-βV spectrin bridges the plasma membrane and cortical lattice in the lateral wall of the auditory outer hair cells

Kirian Legendre1,2,3, Saaid Safieddine1,2,3, Polonca Küssel-Andermann1, Christine Petit1,2,3 and Aziz El-Amraoui1,2,3,*

1 Institut Pasteur, Unité de Génétique et Physiologie de l'Audition, 25 rue du Dr Roux, 75015 Paris, France
2 INSERM UMRS587, F75015 Paris, France
3 UPMC Paris 06, F75015 Paris, France

* Author for correspondence (e-mail: aziz.el-amraoui{at}pasteur.fr)

Accepted 21 July 2008

The sensitivity and frequency selectivity of the mammalian cochlea involves a mechanical amplification process called electromotility, which requires prestin-dependent length changes of the outer hair cell (OHC) lateral wall in response to changes in membrane electric potential. The cortical lattice, the highly organized cytoskeleton underlying the OHC lateral plasma membrane, is made up of F-actin and spectrin. Here, we show that {alpha}II and two of the five β-spectrin subunits, βII and βV, are present in OHCs. βII spectrin is restricted to the cuticular plate, a dense apical network of actin filaments, whereas βV spectrin is concentrated at the cortical lattice. Moreover, we show that {alpha}II-βV spectrin directly interacts with F-actin and band 4.1, two components of the OHC cortical lattice. βV spectrin is progressively recruited into the cortical lattice between postnatal day 2 (P2) and P10 in the mouse, in parallel with prestin membrane insertion, which itself parallels the maturation of cell electromotility. Although βV spectrin does not directly interact with prestin, we found that addition of lysates derived from mature auditory organs, but not from the brain or liver, enables βV spectrin–prestin interaction. Using this assay, βV spectrin, via its PH domain, indirectly interacts with the C-terminal cytodomain of prestin. We conclude that the cortical network involved in the sound-induced electromotility of OHCs contains {alpha}II-βV spectrin, and not the conventional {alpha}II-βII spectrin.

Key words: βV spectrin, Prestin, Outer hair cell, Cortical lattice, Electromotility


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Spectrin defies convention in OHCs

JCS 2008 121: 2005. [Full Text]  



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K. Legendre, S. Safieddine, P. Kussel-Andermann, C. Petit, and A. El-Amraoui
{alpha}II-{beta}V spectrin bridges the plasma membrane and cortical lattice in the lateral wall of the auditory outer hair cells
Development, November 1, 2008; 135(21): e1 - e1.
[Full Text]


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