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First published online 22 January 2008
doi: 10.1242/jcs.015826

Journal of Cell Science 121, 428-436 (2008)
Published by The Company of Biologists 2008
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Research Article

Tetrahymena IFT122A is not essential for cilia assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body

Che-Chia Tsao and Martin A. Gorovsky*

Department of Biology, University of Rochester, Rochester, NY 14627, USA

* Author for correspondence (e-mail: goro{at}mail.rochester.edu)

Accepted 12 November 2007

Intraflagellar transport (IFT) moves multiple protein particles composed of two biochemically distinct complexes, IFT-A and IFT-B, bi-directionally within cilia and is essential for cilia assembly and maintenance. We identified an ORF from the Tetrahymena macronuclear genome sequence, encoding IFT122A, an ortholog of an IFT-A complex protein. Tetrahymena IFT122A is induced during cilia regeneration, and epitope-tagged Ift122Ap could be detected in isolated cilia. IFT122A knockout cells still assembled cilia, albeit with lower efficiency, and could regenerate amputated cilia. Ift172p and Ift88p, two IFT-B complex proteins that localized mainly to basal bodies and along the cilia in wild-type cells, became preferentially enriched at the ciliary tips in IFT122A knockout cells. Our results indicate that Tetrahymena IFT122A is not required for anterograde transport-dependent ciliary assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body.

Key words: Intraflagellar transport, Cilia/flagella, Ciliary assembly, WD domain protein, Gene targeting


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