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First published online 5 February 2008
doi: 10.1242/jcs.018937

Journal of Cell Science 121, 578-589 (2008)
Published by The Company of Biologists 2008
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Research Article

Xenopus NEDD1 is required for microtubule organization in Xenopus egg extracts

Lingling Liu* and Christiane Wiese{ddagger}

Department of Biochemistry, University of Wisconsin – Madison, 433 Babcock Drive, Madison, WI, USA

{ddagger} Author for correspondence (e-mail: wiese{at}biochem.wisc.edu)

Accepted 29 November 2007

The centrosome serves as the major microtubule-nucleating and -organizing center in animal cells. It is composed of hundreds of proteins. The molecular details of how centrosomal proteins contribute to centrotome function are only beginning to emerge. Members of the neuron-precursor-cell-expressed developmentally downregulated protein 1 (NEDD1) family of conserved proteins have recently been implicated in recruiting {gamma}-tubulin and its associated proteins, which together make up the {gamma}-tubulin ring complex ({gamma}TuRC), to the centrosome. Human NEDD1 and its Drosophila ortholog Dgp71WD are WD-repeat proteins that interact with the {gamma}TuRC. Experimental knockdown of human NEDD1 was recently shown to result in loss of {gamma}-tubulin from the centrosome. By contrast, however, Dgp71WD knockdown has no effect on targeting the {gamma}TuRC to the centrosome in flies. Using Xenopus egg extracts, we show that Xenopus NEDD1 is mostly dispensable for targeting {gamma}-tubulin to centrosomes, but that microtubule organization is disrupted in NEDD1-depleted extracts. We show that NEDD1 exists in a complex that is distinct from the {gamma}TuRC, suggesting that NEDD1 may not be a bona fide subunit of the Xenopus {gamma}TuRC. We propose that the main function of NEDD1 in Xenopus is in microtubule organization.

Key words: Xenopus NEDD1, {gamma}-tubulin, {gamma}TuRC, Microtubule organization, Centrosome






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