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First published online April 3, 2008
doi: 10.1242/10.1242/jcs.025833

Journal of Cell Science 121, 1264-1274 (2008)
Published by The Company of Biologists 2008
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Research Article

Internalization of LDL-receptor superfamily yolk-protein receptors during mosquito oogenesis involves transcriptional regulation of PTB-domain adaptors

Sanjay K. Mishra1,*, Anupma Jha1,*, Amie L. Steinhauser1, Vladimir A. Kokoza2, Charles H. Washabaugh1, Alexander S. Raikhel2, Woodbridge A. Foster3 and Linton M. Traub1,{ddagger}

1 Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
2 Department of Entomology, University of California, Riverside, CA 92521, USA
3 Department of Entomology, Ohio State University, Columbus, OH 43210, USA

{ddagger} Author for correspondence (e-mail: traub{at}pitt.edu)

Accepted 28 January 2008

In the anautogenous disease vector mosquitoes Anopheles gambiae and Aedes aegypti, egg development is nutritionally controlled. A blood meal permits further maturation of developmentally repressed previtellogenic egg chambers. This entails massive storage of extraovarian yolk precursors by the oocyte, which occurs through a burst of clathrin-mediated endocytosis. Yolk precursors are concentrated at clathrin-coated structures on the oolemma by two endocytic receptors, the vitellogenin and lipophorin receptors. Both these mosquito receptors are members of the low-density-lipoprotein-receptor superfamily that contain FxNPxY-type internalization signals. In mammals, this tyrosine-based signal is not decoded by the endocytic AP-2 adaptor complex directly. Instead, two functionally redundant phosphotyrosine-binding domain adaptors, Disabled 2 and the autosomal recessive hypercholesterolemia protein (ARH) manage the internalization of the FxNPxY sorting signal. Here, we report that a mosquito ARH-like protein, which we designate trephin, possess similar functional properties to the orthologous vertebrate proteins despite engaging AP-2 in an atypical manner, and that mRNA expression in the egg chamber is strongly upregulated shortly following a blood meal. Temporally regulated trephin transcription and translation suggests a mechanism for controlling yolk uptake when vitellogenin and lipophorin receptors are expressed and clathrin coats operate in previtellogenic ovaries.

Key words: LDL receptor, Adaptor, Clathrin-mediated endocytosis, Transcriptional regulation, Yolk protein


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Trephin takes up the yolk

JCS 2008 121: 804. [Full Text]  





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