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First published online 5 May 2009
doi: 10.1242/jcs.041798

Journal of Cell Science 122, 1747-1758 (2009)
Published by The Company of Biologists 2009
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Research Article

Roles of the Drosophila NudE protein in kinetochore function and centrosome migration

Alan Wainman1,*, Jacklyn Creque2,*, Byron Williams2, Erika V. Williams2, Silvia Bonaccorsi1, Maurizio Gatti1 and Michael L. Goldberg2,{ddagger}

1 Instituto Pasteur Fondazione Cenci Bolognetti and Istituto di Biologia e Patologia Molecolari del CNR, Dipartimento di Genetica e Biologia Molecolare, Sapienza, Università di Roma, 00185 Rome, Italy
2 Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA

{ddagger} Author for correspondence (e-mail: mlg11{at}cornell.edu)

Accepted 16 February 2009

We examined the distribution of the dynein-associated protein NudE in Drosophila larval brain neuroblasts and spermatocytes, and analyzed the phenotypic consequences of a nudE null mutation. NudE can associate with kinetochores, spindles and the nuclear envelope. In nudE mutant brain mitotic cells, centrosomes are often detached from the poles. Moreover, the centrosomes of mutant primary spermatocytes do not migrate from the cell cortex to the nuclear envelope, establishing a new role for NudE. In mutant neuroblasts, chromosomes fail to congress to a tight metaphase plate, and cell division arrests because of spindle assembly checkpoint (SAC) activation. The targeting of NudE to mitotic kinetochores requires the dynein-interacting protein Lis1, and surprisingly Cenp-meta, a Drosophila CENP-E homolog. NudE is non-essential for the targeting of all mitotic kinetochore components tested. However, in the absence of NudE, the `shedding' of proteins off the kinetochore is abrogated and the SAC cannot be turned off, implying that NudE regulates dynein function at the kinetochore.

Key words: Mitosis, Meiosis, Kinetochores, Centrosomes, Spindle assembly checkpoint, Dynein


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Cell division – a need for NudE

JCS 2009 122: 1102. [Full Text]  





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