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First published online 26 May 2009
doi: 10.1242/jcs.049080

Journal of Cell Science 122, 2055-2063 (2009)
Published by The Company of Biologists 2009
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Research Article

MER3 is required for normal meiotic crossover formation, but not for presynaptic alignment in rice

Kejian Wang1, Ding Tang1, Mo Wang1, Jufei Lu2, Hengxiu Yu2, Jiafan Liu2, Baoxiang Qian1, Zhiyun Gong2, Xin Wang2, Jianmin Chen2, Minghong Gu2 and Zhukuan Cheng1,*

1 State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
2 Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, China

* Author for correspondence (e-mail: zkcheng{at}genetics.ac.cn)

Accepted 19 March 2009

MER3, a ZMM protein, is required for the formation of crossovers in Saccharomyces cerevisiae and Arabidopsis. Here, MER3, the first identified ZMM gene in a monocot, is characterized by map-based cloning in rice (Oryza sativa). The null mutation of MER3 results in complete sterility without any vegetative defects. Cytological analyses show that chiasma frequency is reduced dramatically in mer3 mutants and the remaining chiasmata distribute randomly among different pollen mother cells, implying possible coexistence of two kinds of crossover in rice. Immunocytological analyses reveal that MER3 only exists as foci in prophase I meiocytes. In addition, MER3 does not colocalize with PAIR2 at the beginning of prophase I, but locates on one end of PAIR2 fragments at later stages, whereas MER3 foci merely locate on one end of REC8 fragments when signals start to be seen in early prophase I. The normal loading of PAIR2 and REC8 in mer3 implies that their loading is independent of MER3. On the contrary, the absence of MER3 signal in pair2 mutants indicates that PAIR2 is essential for the loading and further function of MER3.

Key words: Rice, Meiosis, MER3, Crossover


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© The Company of Biologists Ltd 2009