spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 16 June 2009
doi: 10.1242/jcs.042861

Journal of Cell Science 122, 2393-2401 (2009)
Published by The Company of Biologists 2009
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow All Versions of this Article:
jcs.042861v1
122/14/2393    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Lin, H.-C.
Right arrow Articles by Chien, C.-T.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lin, H.-C.
Right arrow Articles by Chien, C.-T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Cul4 and DDB1 regulate Orc2 localization, BrdU incorporation and Dup stability during gene amplification in Drosophila follicle cells

Hsiu-Chen Lin1,2, June-Tai Wu3,4, Bertrand Chin-Ming Tan5 and Cheng-Ting Chien1,2,*

1 Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan
2 Institute of Molecular Biology, Academia Sinica, 128, Sec No. 2, Academia Road, Taipei 115, Taiwan
3 Department of Medical Research, National Taiwan University Hospital, No. 7, Chung San South Road, Taipei 115, Taiwan
4 Gratuate Institute of Molecular Medicine, Medical College, National Taiwan University. No. 1, Ren-Ai Road Section 1., Taipei 100, Taiwan
5 Department of Life Science, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-San, Tao-Yuan 333, Taiwan

* Author for correspondence (e-mail: ctchien{at}gate.sinica.edu.tw)

Accepted 1 April 2009

In higher eukaryotes, the pre-replication complex (pre-RC) component Cdt1 is the major regulator in licensing control for DNA replication. The Cul4-DDB1-based ubiquitin ligase mediates Cdt1 ubiquitylation for subsequent proteolysis. During the initiation of chorion gene amplification, Double-parked (Dup), the Drosophila ortholog of Cdt1, is restricted to chorion gene foci. We found that Dup accumulated in nuclei in Cul4 mutant follicle cells, and the accumulation was less prominent in DDB1 mutant cells. Loss of Cul4 or DDB1 activity in follicle cells also compromised chorion gene amplification and induced ectopic genomic DNA replication. The focal localization of Orc2, a subunit of the origin recognition complex, is frequently absent in Cul4 mutant follicle cells. Therefore, Cul4 and DDB1 have differential functions during chorion gene amplification.

Key words: Cul4, DDB1, Proliferation, DNA replication, pre-RC, Dup, Cdt1, Orc2, Chorion gene amplification


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?




© The Company of Biologists Ltd 2009