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First published online July 1, 2009
doi: 10.1242/10.1242/jcs.049999

Journal of Cell Science 122, 2464-2472 (2009)
Published by The Company of Biologists 2009
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Research Article

Nuclear shape, growth and integrity in the closed mitosis of fission yeast depend on the Ran-GTPase system, the spindle pole body and the endoplasmic reticulum

Yanira Gonzalez1,*, Kristen Meerbrey1,2,*, Jennifer Chong1, Yoshihiro Torii1,2, Neal N. Padte3 and Shelley Sazer1,2,4,{ddagger}

1 Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
2 Graduate Program in Cell and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
3 Microbiology Department, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
4 Department of Molecular and Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA

{ddagger} Author for correspondence (e-mail: ssazer{at}bcm.edu)

Accepted 14 April 2009

The double lipid bilayer of the nuclear envelope (NE) remains intact during closed mitosis. In the fission yeast Schizosaccharomyces pombe, the intranuclear mitotic spindle has envelope-embedded spindle pole bodies (SPB) at its ends. As the spindle elongates and the nucleus divides symmetrically, nuclear volume remains constant but nuclear area rapidly increases by 26%. When Ran-GTPase function is compromised in S. pombe, nuclear division is strikingly asymmetrical and the newly synthesized SPB is preferentially associated with the smaller nucleus, indicative of a Ran-dependent SPB defect that interferes with symmetrical nuclear division. A second defect, which specifically influences the NE, results in breakage of the NE upon spindle elongation. This defect, but not asymmetric nuclear division, is partially rescued by slowing spindle elongation, stimulating endoplasmic reticulum (ER) proliferation or changing conformation of the ER membrane. We propose that redistribution of lipid within the ER-NE network is crucial for mitosis-specific NE changes in both open and closed mitosis.

Key words: Ran GTPase, Nuclear division, Fission yeast, Endoplasmic reticulum, Spindle pole body


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JCS 2009 122: 1402. [Full Text]  





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