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First published online 6 January 2009
doi: 10.1242/jcs.026179

Journal of Cell Science 122, 401-413 (2009)
Published by The Company of Biologists 2009
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Research Article

Dynamic complexes of A-type lamins and emerin influence adipogenic capacity of the cell via nucleocytoplasmic distribution of β-catenin

Katarzyna Tilgner*, Kamila Wojciechowicz*, Colin Jahoda, Christopher Hutchison and Ewa Markiewicz{ddagger}

The School of Biological and Biomedical Sciences, The University of Durham, South Road, Durham DH1 3LE, UK

{ddagger} Author for correspondence (e-mail: e.m.markiewicz{at}durham.ac.uk)

Accepted 22 October 2008

It is well documented that adipogenic differentiation of the cell is associated with downregulation of Wnt/β-catenin signalling. Using preadipocytes and dermal fibroblasts, we have found that activation of the adipogenic program was associated with marked changes in the expression of nuclear β-catenin-interacting partners, emerin and lamins A/C, to influence expression and activation of peroxisome proliferators-activated receptors {gamma} (PPAR{gamma}). In addition, silencing of protein expression with siRNA revealed that β-catenin and emerin influenced each other's levels of expression and the onset of adipogenesis, suggesting that changes in the expression of nuclear lamina proteins were intimately linked to the stability of β-catenin. By contrast, dermal fibroblasts, which are emerin null, demonstrated increased nuclear accumulation of stable β-catenin and constant lamin expression. This was also associated with an unusual adipogenic capacity of the cells, with adipogenesis occurring in the presence of activated β-catenin but declining upon silencing of the protein expression with siRNA. We propose that the process of adipogenesis is affected by a dynamic link between complexes of emerin and lamins A/C at the nuclear envelope and nucleocytoplasmic distribution of β-catenin, to influence cellular plasticity and differentiation.

Key words: Nuclear lamina, β-catenin, Adipogenesis


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