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First published online 27 January 2009
doi: 10.1242/jcs.042382

Journal of Cell Science 122, 489-498 (2009)
Published by The Company of Biologists 2009
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Research Article

Involvement of SIRT7 in resumption of rDNA transcription at the exit from mitosis

Alice Grob1, Pascal Roussel1, Jane E. Wright2, Brian McStay2, Danièle Hernandez-Verdun1 and Valentina Sirri1,*

1 Institut Jacques Monod, UMR 7592 CNRS/Universités Paris 6 et 7, 2 Place Jussieu, 75251 Paris Cedex 05, France
2 Biomedical Research Center, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, Scotland, UK

* Author for correspondence (e-mail: sirri{at}ijm.jussieu.fr)

Accepted 14 October 2008

Sirtuins, also designated class III histone deacetylases, are implicated in the regulation of cell division, apoptosis, DNA damage repair, genomic silencing and longevity. The nucleolar Sirtuin7 (SIRT7) was reported to be involved in the regulation of ribosomal gene (rDNA) transcription, but there are no data concerning the regulation of SIRT7 during the cell cycle. Here we have analyzed the behavior of endogenous SIRT7 during mitosis, while rDNA transcription is repressed. SIRT7 remains associated with nucleolar organizer regions, as does the RNA polymerase I machinery. SIRT7 directly interacts with the rDNA transcription factor UBF. Moreover, SIRT7 is phosphorylated via the CDK1-cyclin B pathway during mitosis and dephosphorylated by a phosphatase sensitive to okadaic acid at the exit from mitosis before onset of rDNA transcription. Interestingly, dephosphorylation events induce a conformational modification of the carboxy-terminal region of SIRT7 before the release of mitotic repression of rDNA transcription. As SIRT7 activity is required to resume rDNA transcription in telophase, we propose that this conformational modification regulates onset of rDNA transcription.

Key words: Sirtuins, Nucleolus, Cell cycle, NOR, CDK1


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