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First published online 10 February 2009
doi: 10.1242/jcs.032102

Journal of Cell Science 122, 687-695 (2009)
Published by The Company of Biologists 2009
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Research Article

Apical membrane maturation and cellular rosette formation during morphogenesis of the zebrafish lateral line

David Hava1,*, Ulrike Forster1,*, Miho Matsuda2, Shuang Cui3, Brian A. Link3, Jenny Eichhorst4, Burkhard Wiesner4, Ajay Chitnis2 and Salim Abdelilah-Seyfried1,{ddagger}

1 Max Delbrück Center (MDC) for Molecular Medicine, D-13125 Berlin, Germany
2 Unit on Vertebrate Neural Development, Laboratory of Molecular Genetics, NIH/NICHD, Bethesda, MD 20892, USA
3 Medical College of Wisconsin, Department of Cell Biology, Neurobiology and Anatomy, Milwaukee, WI 53226, USA
4 Leibniz Institute for Molecular Pharmacology (FMP), D-13125 Berlin, Germany

{ddagger} Author for correspondence (e-mail: salim{at}mdc-berlin.de)

Accepted 14 November 2008

Tissue morphogenesis and cell sorting are major forces during organ development. Here, we characterize the process of tissue morphogenesis within the zebrafish lateral line primordium, a migratory sheet of cells that gives rise to the neuromasts of the posterior lateral line organ. We find that cells within this epithelial tissue constrict actin-rich membranes and enrich apical junction proteins at apical focal points. The coordinated apical membrane constriction in single Delta D-positive hair cell progenitors and in their neighbouring prospective support cells generates cellular rosettes. Live imaging reveals that cellular rosettes subsequently separate from each other and give rise to individual neuromasts. Genetic analysis uncovers an involvement of Lethal giant larvae proteins in the maturation of apical junction belts during cellular rosette formation. Our findings suggest that apical constriction of cell membranes spatially confines regions of strong cell-cell adhesion and restricts the number of tightly interconnected cells into cellular rosettes, which ensures the correct deposition of neuromasts during morphogenesis of the posterior lateral line organ.

Key words: Lethal giant larvae 2, Cell polarity, Lateral line organ, Protein kinase C{iota}, Heart and soul, Adhesion


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