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First published online 10 February 2009
doi: 10.1242/jcs.040345

Journal of Cell Science 122, 696-705 (2009)
Published by The Company of Biologists 2009
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Research Article

Yersinia enterocolitica differentially modulates RhoG activity in host cells

Bernhard Roppenser, Anja Röder, Moritz Hentschke, Klaus Ruckdeschel and Martin Aepfelbacher*

Institut für Medizinische Mikrobiologie, Virologie und Hygiene, Universitätsklinikum Hamburg-Eppendorf, Martinistraβe 52, 20246 Hamburg, Germany

* Author for correspondence (e-mail: m.aepfelbacher{at}uke.uni-hamburg.de)

Accepted 10 November 2008

Pathogenic bacteria of the genus Yersinia (Y. pestis, Y. enterocolitica and Y. pseudotuberculosis) have evolved numerous virulence factors (termed a stratagem) to manipulate the activity of Rho GTPases. Here, we show that Y. enterocolitica modulates RhoG, an upstream regulator of other Rho GTPases. At the contact site of virulent Y. enterocolitica and host cells, we could visualise spatiotemporally organised activation and deactivation of RhoG. On the one hand, the β1-integrin clustering protein Invasin on the bacterial surface was found to activate RhoG and this promoted cell invasion. On the other hand, active RhoG was downregulated by the type III secretion system effector YopE acting as a GTPase-activating protein (GAP). YopE localised to Golgi and endoplasmic reticulum, and this determined its specificity for RhoG and other selected Rho GTPases. RhoG and its downstream effector module Elmo/Dock180 controlled both Rac1 activation by Invasin and Rac1 deactivation by YopE. We propose that RhoG is a central target of the Yersinia stratagem and a major upstream regulator of Rac1 during different phases of the Yersinia infection cycle.

Key words: Yersinia enterocolitica, RhoG, YopE, RhoGAP, Elmo, Dock180, Invasin


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This article has been cited by other articles:


Home page
 Infect. Immun.Home page
S. Mohammadi and R. R. Isberg
Yersinia pseudotuberculosis Virulence Determinants Invasin, YopE, and YopT Modulate RhoG Activity and Localization
Infect. Immun., November 1, 2009; 77(11): 4771 - 4782.
[Abstract] [Full Text] [PDF]


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