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First published online 19 March 2009
doi: 10.1242/jcs.045740

Journal of Cell Science 122, 1081-1090 (2009)
Published by The Company of Biologists 2009
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Research Article

Three-dimensional cellular architecture of the flagellar pocket and associated cytoskeleton in trypanosomes revealed by electron microscope tomography

Sylvain Lacomble1, Sue Vaughan1, Catarina Gadelha1, Mary K. Morphew2, Michael K. Shaw1, J. Richard McIntosh2 and Keith Gull1,*

1 Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
2 Laboratory for 3-D Electron Microscopy of Cells, Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA

* Author for correspondence (e-mail: keith.gull{at}path.ox.ac.uk)

Accepted 8 December 2008

This study uses electron tomography linked to a variety of other EM methods to provide an integrated view of the flagellar pocket and basal body area of the African trypanosome procyclic trypomastigote. We reveal the pocket as an asymmetric membranous `balloon' with two boundary structures. One of these – the collar – defines the flagellum exit point. The other defines the entry point of the flagellum into the pocket and consists of both an internal transitional fibre array and an external membrane collarette. A novel set of nine radial fibres is described in the basal body proximal zone. The pocket asymmetry is invariably correlated with the position of the probasal body and Golgi. The neck region, just distal to the flagellum exit site, is a specialised area of membrane associated with the start of the flagellum attachment zone and signifies the point where a special set of four microtubules, nucleated close to the basal bodies, joins the subpellicular array. The neck region is also associated with the single Golgi apparatus of the cell. The flagellar exit point interrupts the subpellicular microtubule array with discrete endings of microtubules at the posterior side. Overall, our studies reveal a highly organised, yet dynamic, area of cytoplasm and will be informative in understanding its function.

Key words: Trypanosome, Flagellum, Flagellar pocket, Basal body, Electron tomography, Golgi, Microtubule, Cytoskeleton, Secretion


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 Proc. Natl. Acad. Sci. USAHome page
C. Gadelha, S. Rothery, M. Morphew, J. R. McIntosh, N. J. Severs, and K. Gull
Membrane domains and flagellar pocket boundaries are influenced by the cytoskeleton in African trypanosomes
PNAS, October 13, 2009; 106(41): 17425 - 17430.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2009