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First published online December 16, 2009
doi: 10.1242/10.1242/jcs.055889

Journal of Cell Science 123, 40-50 (2010)
Published by The Company of Biologists 2010
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Research Article

Human RBMY regulates germline-specific splicing events by modulating the function of the serine/arginine-rich proteins 9G8 and Tra2-β

Natacha Dreumont1,2,3,4, Cyril F. Bourgeois1,2,3,4,*, Fabrice Lejeune5, Yilei Liu6, Ingrid E. Ehrmann6, David J. Elliott6 and James Stévenin1,2,3,4,{ddagger}

1 IGBMC Department of Functional Genomics, Illkirch, France
2 INSERM U964, Illkirch, France
3 CNRS UMR 7104, Illkirch, France
4 University of Strasbourg, Strasbourg, France
5 Institut Pasteur de Lille, Lille, France
6 Institute of Human Genetics, University of Newcastle, International Center for Life, Central Parkway, Newcastle upon Tyne, NE1 3BZ, England

{ddagger} Author for correspondence (stevenin{at}igbmc.fr)

Accepted 15 October 2009

RBMY is a male germline RNA binding protein and potential alternative splicing regulator, but the lack of a convenient biological system has made its cellular functions elusive. We found that human RBMY fused to green fluorescent protein was strictly nuclear in transfected cells, but spatially enriched in areas around nuclear speckles with some components of the exon junction complex (EJC). Human RBMY (hRBMY) and the EJC components Magoh and Y14 also physically interacted but, unlike these two proteins, hRBMY protein did not shuttle to the cytoplasm. In addition, it relocalised into nucleolar caps after inhibition of RNA polymerase II transcription. Protein interactions were also detected between RBMY and splicing factors 9G8 and transformer-2 protein homolog β (Tra2-β), mediated by multiple regions of the RBMY protein that contain serine/arginine-rich dipeptides, but not by the single region lacking such dipeptides. These interactions modulated the splicing of several pre-mRNAs regulated by 9G8 and Tra2-β. Importantly, ectopic expression of hRBMY stimulated the inclusion of a testis-enriched exon from the Acinus gene, whereas 9G8 and Tra2-β repressed this exon. We propose that hRBMY associates with regions of the nucleus enriched in nascent RNA and participates in the regulation of specific splicing events in the germline by modulating the activity of constitutively expressed splicing factors.

Key words: hRBMY, Alternative splicing, Testis, Exon-junction complex, SR proteins


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