|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 13, 337-357, Copyright © 1973 by Company of Biologists
Submitted on January 3, 1973
1 Pacific Biomedical Research Center, University of Hawaii, 41 Ahui Street, Honolulu, Hawaii 96813, U.S.A.
Sea-urchin sperm were extracted with o.5 M KCl for 45 s at room temperature in the presence of Triton X-100, and then transferred to reactivating solution containing 1 mM ATP. The flagellar beat frequency of these KCl-extracted sperm (16 beats/s) was only about half that of control Triton-extracted sperm that had not been exposed to 0.5 M KCl (31 beats/s), although the form of their bending waves was not significantly altered. Examination by electron microscopy showed that the extraction with 0.5 M KCl removed the majority of the outer arms from the doublet tubules, leaving the inner arms apparently intact. By varying the duration of the KCl-extraction, it was shown that the rate of decrease in beat frequency paralleled the rate of disappearance of the arms. Prolonging the extraction time beyond 45 s at room temperature, or 4 min at o °C, had little further effect on beat frequency. ATPase measurements suggested that 6o-65% of the dynein in the original axonemes had been solubilized when the extraction with KCl was permitted to go to completion. These results indicate that the generation and propagation of flagellar bending waves of essentially typical form are not prevented by the removal of the outer row of dynein arms from the doublet tubules. In terms of the sliding filament model of flagellar bending, the results suggest that the rate of sliding between tubules under these conditions is proportional to the number of dynein arms present. The lack of significant change in wave form implies that the total amount of sliding that occurs during each bending cycle is not affected by the reduced number of dynein arms, but is regulated independently in some manner by the elastic forces generated by other structures in the bent axoneme.
Submitted on January 3, 1973
This article has been cited by other articles:
|
|
 |
|
  J. Freshour, R. Yokoyama, and D. R. Mitchell Chlamydomonas Flagellar Outer Row Dynein Assembly Protein Oda7 Interacts with Both Outer Row and I1 Inner Row Dyneins J. Biol. Chem., February 23, 2007; 282(8): 5404 - 5412. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. R. Dawe, H. Farr, and K. Gull Centriole/basal body morphogenesis and migration during ciliogenesis in animal cells J. Cell Sci., January 1, 2007; 120(1): 7 - 15. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gadelha, B. Wickstead, P. G. McKean, and K. Gull Basal body and flagellum mutants reveal a rotational constraint of the central pair microtubules in the axonemes of trypanosomes J. Cell Sci., June 15, 2006; 119(12): 2405 - 2413. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K Ashizawa, G J Wishart, S Katayama, D Takano, M Maeda, E Arakawa, and Y Tsuzuki Effects of calpain and Rho-kinase inhibitors on the acrosome reaction and motility of fowl spermatozoa in vitro Reproduction, January 1, 2006; 131(1): 71 - 79. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. B. Lindemann Structural-Functional Relationships of the Dynein, Spokes, and Central-Pair Projections Predicted from an Analysis of the Forces Acting within a Flagellum Biophys. J., June 1, 2003; 84(6): 4115 - 4126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Woolley Studies on the eel sperm flagellum. I. The structure of the inner dynein arm complex J. Cell Sci., January 1, 1997; 110(1): 85 - 94. [Abstract] [PDF]
|
|
|
|
|
|
S Ishijima, M Kubo-Irie, H Mohri, and Y Hamaguchi Calcium-dependent bidirectional power stroke of the dynein arms in sea urchin sperm axonemes J. Cell Sci., January 12, 1996; 109(12): 2833 - 2842. [Abstract] [PDF]
|
|
|
|
|
|
C Shingyoji and K Takahashi Cyclical bending movements induced locally by successive iontophoretic application of ATP to an elastase-treated flagellar axoneme J. Cell Sci., January 4, 1995; 108(4): 1359 - 1369. [Abstract] [PDF]
|
|
|
|
|
|
E Yokota and I Mabuchi C/A dynein isolated from sea urchin sperm flagellar axonemes. Enzymatic properties and interaction with microtubules J. Cell Sci., January 2, 1994; 107(2): 353 - 361. [Abstract] [PDF]
|
|
